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[Preprint]. 2024 Oct 18:2024.10.10.24315212.
doi: 10.1101/2024.10.10.24315212.

Multiplex PCR detection of enteric pathogens in a community-based birth cohort in Ecuador: comparison of xTAG-GPP and TaqMan array card assays

Stuart Torres Ayala  1 , Lesly Simbaña Vivanco  1 , Nikolina Walas  2 , Kelsey Jesser  3 , Nicolette A Zhou  3 , Christine S Fagnant-Sperati  3 , Hadley R Burroughs  4 , Gwenyth O Lee  5 , Joseph N S Eisenberg  6 , Gabriel Trueba  1 , Karen Levy  3 , Benjamin F Arnold  4   7   8
Affiliations

Multiplex PCR detection of enteric pathogens in a community-based birth cohort in Ecuador: comparison of xTAG-GPP and TaqMan array card assays

Stuart Torres Ayala et al. medRxiv. .

Update in

Abstract

We compared the performance of two multiplex platforms, Luminex xTAG Gastrointestinal Pathogen Panel® and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

Keywords: bacteria; enteric pathogens; multiplex PCR; protozoans; viruses.

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Conflict of interest statement

Competing interests: The authors declare no competing interests.

Figures

Figure 1:
Figure 1:. Infection prevalence for 14 enteric pathogens measured by Luminex xTAG Gastrointestinal Panel (GPP) and TaqMan Array Card (TAC) assays.
Analysis includes 154 samples from children ages 6 to 18 months in Esmeraldas Province, Ecuador 2022–2023. An asterisk indicates McNemar’s P<0.05 for difference between the two assays. Supplemental Table 3 includes numerical estimates. Created with script: https://osf.io/4dteq.
See this image and copyright information in PMC

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