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. 2024 Dec;56(6):657-668.
doi: 10.1007/s10863-024-10042-x. Epub 2024 Oct 19.

N6-methyladenosine (m6A) reader HNRNPA2B1 accelerates the cervical cancer cells aerobic glycolysis

Mengke Wen  1 , Na Yi  2 , Bulabiyamu Mijiti  3 , Shihong Zhao  1 , Guqun Shen  4
Affiliations

N6-methyladenosine (m6A) reader HNRNPA2B1 accelerates the cervical cancer cells aerobic glycolysis

Mengke Wen et al. J Bioenerg Biomembr. 2024 Dec.

Abstract

N6-methyladenosine (m6A) modification is, a more common epigenetic modification, mainly found in mRNA. More and more researches have shown the important functions of m6A on human cancers. This study seeks to explore the role of hnRNPA2B1 and m6A-dependent mechanism in cervical cancer. Elevated hnRNPA2B1 indicated the poor prognosis of cervical cancer patients. Enforced hnRNPA2B1 reduced the apoptosis, and accelerated the proliferation and migration of cervical cancer cells in vitro. Besides, hnRNPA2B1 promoted the aerobic glycolysis of cervical cancer cells, including the lactate secretion, glucose uptake, ATP production, extracellular acidification rate (ECAR) and oxygen consumption rate (OCR). LDHA was found as the downstream target of hnRNPA2B1 by m6A site. Moreover, hnRNPA2B1 enhanced the mRNA stability of LDHA through m6A-dependent manner. LDHA inhibitor (FX-11) could reverse the effect of hnRNPA2B1. Taken together, the data revealed that hnRNPA2B1 promoted the proliferation, migration and aerobic glycolysis of cervical cancer cells by m6A/LDHA-dependent manner. These findings might bring a new idea for cervical cancer treatment.

Keywords: Aerobic glycolysis; Cervical cancer; LDHA; hnRNPA2B1.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: Written informed consent was obtained from each patient, and the study had been approved by the Ethical Committee of Xinjiang Medical University. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.

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