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. 2024 Oct 19;14(1):24582.
doi: 10.1038/s41598-024-76723-0.

Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves

Xiaochen Zhu  1   2 , Penny Miller-Ezzy  2 , Tony Hall  3 , Youhong Tang  1 , Jianguang Qin  1 , Yingying Zhao  4 , Xiaoxu Li  5
Affiliations

Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves

Xiaochen Zhu et al. Sci Rep. .

Abstract

Larval cryopreservation techniques have been established in Pacific oysters and Mediterranean mussels. Although initial cryopreservation protocols for both species differed slightly in Ficoll PM 70 (FIC) concentration, better post-thaw larval survivability was produced in mussels than oysters. Furthermore, the post-thaw survivability in the latter could be improved significantly by the addition of lipids and antioxidants to the cryoprotectant agent. These findings have generated a unique opportunity to investigate the cryo-functions of both endogenous and exogenous fatty acids (FAs) in bivalves as mammalian studies indicated that lipid compositions could contribute the interspecific difference in gamete and embryo cryopreservation. Our study showed that the higher survivability of post-thaw larvae in mussels (65% vs. 34% in oysters) could be attributed to their higher proportion of total polyunsaturated fatty acids (PUFAs), especially C22:6, and higher resistance of FA profiles to cryopreservation. In oysters, on the other hand, their FA profiles were very sensitive to cryopreservation, with saturated FAs and PUFAs being significantly increased (from 37 to 41%) and decreased (from 35% to ~ 32%), respectively. Although exogenous lipids could significantly improve the survivability of post-thaw oyster larvae from 34 to 51%, their supplementation did not alter the response pattern of endogenous FA profiles to cryopreservation.

Keywords: Magallana gigas; Mytilus galloprovincialis; Fatty acid profile response; Larval cryopreservation; Polyunsaturated fatty acids.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Survival rates of fresh controls (A) and relative survival rates of post-thaw larvae (B) at different ages in Mediterranean mussels and Pacific oysters (n = 3). ns indicates no significant difference between fresh oysters and mussels. Different lowercase or uppercase letters indicate significant differences between treatments within the same age in oysters.
See this image and copyright information in PMC

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