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. 2025 Jan;24(1):193-200.
doi: 10.1016/j.jcf.2024.10.003. Epub 2024 Oct 20.

Evidence for secondary ciliary dyskinesia in patients with cystic fibrosis

Romane Bonhiver  1 , Noemie Bricmont  2 , Maud Pirotte  3 , Marc-Antoine Wuidart  4 , Justine Monseur  5 , Lionel Benchimol  6 , Anne-Lise Poirrier  6 , Catherine Moermans  7 , Doriane Calmés  8 , Florence Schleich  7 , Renaud Louis  7 , Marie-Christine Seghaye  9 , Céline Kempeneers  2
Affiliations
Free article

Evidence for secondary ciliary dyskinesia in patients with cystic fibrosis

Romane Bonhiver et al. J Cyst Fibros. 2025 Jan.
Free article

Abstract

Background: Mucociliary clearance (MCC) impairment can be due to mucus abnormalities or to a ciliary dysfunction, which can be innate, or secondary to infection and/or inflammation. In cystic fibrosis (CF), it is well documented that MCC is impaired due to mucus abnormalities, but little is known concerning ciliary beating. This study aimed to confirm that ciliary dyskinesia is present in CF, and if this might be innate or secondary to the chronic infection and/or inflammation.

Methods: Ciliated epithelial samples were obtained by nasal brushing from 51 CF patients, and from 30 healthy subjects. Ciliary beating was evaluated using digital high-speed videomicroscopy at 37 °C, allowing to evaluate ciliary beat frequency (CBF) and the percentage of abnormal beat pattern (CBP); this was repeated after air-liquid interface (ALI) cell culture.

Results: Ciliary dyskinesia was higher in CF patients than in healthy subjects, with a lower CBF and a higher percentage of abnormal CBP. Ciliary dyskinesia, already present in childhood, normalized after ALI cell culture. A chronic airway colonization did not worsen ciliary dyskinesia.

Conclusions: We showed that, in CF, a ciliary dyskinesia, present from childhood, might contribute to the impaired MCC. Our results also found that the abnormal ciliary beating was not associated with a chronic infection, and resolved after ALI cell culture, suggesting that ciliary dyskinesia in CF is not innate, and might be secondary to chronic inflammation.

Keywords: Cilia; Ciliary dyskinesia; Cystic fibrosis; Mucociliary clearance.

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Conflict of interest statement

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Anne-Lise Poirrier reports a relationship with GSK that includes: consulting or advisory and speaking and lecture fees. Anne-Lise Poirrier reports a relationship with Sanofi that includes: consulting or advisory and speaking and lecture fees. Renaud Louis reports a relationship with GSK that includes: consulting or advisory, funding grants, and speaking and lecture fees. Renaud Louis reports a relationship with AstraZeneca that includes: consulting or advisory, funding grants, and speaking and lecture fees. Renaud Louis reports a relationship with Chiesi that includes: funding grants and speaking and lecture fees. Florence Schleich reports a relationship with GSK that includes: consulting or advisory, funding grants. Florence Schleich reports a relationship with AstraZeneca that includes: consulting or advisory, funding grants. Florence Schleich reports a relationship with ALK that includes: consulting or advisory. Florence Schleich reports a relationship with Novartis that includes: consulting or advisory. Celine Kempeneers reports a relationship with Sanofi that includes: consulting or advisory.