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. 2025:2858:159-172.
doi: 10.1007/978-1-0716-4140-8_14.

Aqueous Humor Angiography

Clemens A Strohmaier  1   2 , Fiona McDonnell  3 , Alex S Huang  4
Affiliations

Aqueous Humor Angiography

Clemens A Strohmaier et al. Methods Mol Biol. 2025.

Abstract

Aqueous humor angiography, or aqueous angiography, is an anterior segment imaging technique capable of visualizing the conventional/trabecular aqueous humor outflow pathways. As a translational technique, it is applicable for in vivo imaging in living subjects and ex vivo imaging using whole-globe preparations or anterior segment perfusion setups. Excellent spatial and temporal resolution has enabled insights into the segmental distribution of aqueous humor outflow in physiological conditions as well as after trabecular bypass surgery. In this chapter, we thoroughly describe aqueous humor angiography in various experimental setups. The necessary materials for different settings and their application for in vivo and ex vivo experiments as well as notes on dye choice, dye sequences, and special considerations on performing aqueous humor angiography during surgery are presented.

Keywords: Angiography; Aqueous humor outflow; Glaucoma; Intraocular pressure.

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