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. 2023 Dec 5:8:559.
doi: 10.12688/wellcomeopenres.20298.1. eCollection 2023.

The genome sequence of a cranefly, Tipula unca (Wiedemann, 1817)

Olga Sivell  1 , Judy Webb  1 , Ryan Mitchell  2 , Duncan Sivell  1 ; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of a cranefly, Tipula unca (Wiedemann, 1817)

Olga Sivell et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female Tipula unca (a cranefly; Arthropoda; Insecta; Diptera; Tipulidae). The genome sequence is 692.2 megabases in span. Most of the assembly is scaffolded into 4 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.57 kilobases in length.

Keywords: Diptera; Tipula unca; chromosomal; cranefly; genome sequence.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Tipula unca Wiedemann, 1817.
The specimen (NHMUK014037116; idTipUnca1) used for genome sequencing: a) dorsal view, b) lateral view.
Figure 2.
Figure 2.. Genome assembly of Tipula unca, idTipUnca1.1: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 692,223,074 bp assembly. The distribution of sequence lengths is shown in dark grey with the plot radius scaled to the longest sequence present in the assembly (240,654,870 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 sequence lengths (223,236,985 and 205,201,256 bp), respectively. The pale grey spiral shows the cumulative sequence count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the diptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Tipula%20unca/dataset/idTipUnca1_1/snail.
Figure 3.
Figure 3.. Genome assembly of Tipula unca, idTipUnca1.1: BlobToolKit GC-coverage plot.
Scaffolds are coloured by phylum. Circles are sized in proportion to scaffold length. Histograms show the distribution of scaffold length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Tipula%20unca/dataset/idTipUnca1_1/blob.
Figure 4.
Figure 4.. Genome assembly of Tipula unca, idTipUnca1.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Tipula%20unca/dataset/idTipUnca1_1/cumulative.
Figure 5.
Figure 5.. Genome assembly of Tipula unca, idTipUnca1.1: Hi-C contact map of the idTipUnca1.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=btxqqPz1Tbu1jDC6f4-X4A.
See this image and copyright information in PMC

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