Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul;54 Suppl 1(Suppl 1):S54-S56.
doi: 10.1111/vcp.13397. Epub 2024 Oct 23.

What is your diagnosis: Acute hemolysis in a Limousin bull

Alexandre Bertin  1 , Thomas Bonnet  1 , Matthias Lambert  1 , Emi Ludemann  1 , Fabien Corbière  1   2 , Corine Boucraut  3 , Marie-Noëlle Lucas  4 , Catherine Trumel  1   5
Affiliations

What is your diagnosis: Acute hemolysis in a Limousin bull

Alexandre Bertin et al. Vet Clin Pathol. 2025 Jul.
No abstract available

Keywords: Theileria; bovine; cytology; hemolytic anemia; piroplasm; spleen.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Fine‐needle aspiration of the spleen of a dead bull: Hemorrhagic background, numerous ponctiform or pear‐shaped intra‐erythrocyte inclusions suggestive of piroplasms also visible free on the background, activated and erythrophagocytic macrophage with a few bluish inclusions suggestive of hemosiderin. May–Grunwald–Giemsa, ×100 objective.
FIGURE 2
FIGURE 2
Histopathological sample from the spleen of a dead bull. (A) Diffuse and severely congestive red splenic pulp with altered erythrocytes suggestive of hemolysis, infiltrated by macrophages and activated neutrophils. Macrophages are characterized by the presence of pigments suggestive of hemosiderin. (B) Pale erythrocytes containing basophilic ponctiform inclusions suggestive of piroplasm. Eosin and hematoxylin, (A) ×20 objective; (B) ×63 objective.
FIGURE 3
FIGURE 3
Histopathological sample from the kidney of a dead bull. Some cortical renal tubules are necrotic secondary to the accumulation of dark green‐brown pigments, corresponding to bilirubin ± hemoglobin in the nephrocytes. Eosin and hematoxylin, x10 objective.
See this image and copyright information in PMC

References

    1. Jurković D, Mihaljević Ž, Duvnjak S, Silaghi C, Beck R. First reports of indigenous lethal infection with Anaplasma marginale, Anaplasma bovis and Theileria orientalis in Croatian cattle. Ticks Tick Borne Dis. 2020;11(5):101469. - PubMed
    1. Liu J, Li Z, Liu A, et al. Identification and isolation of pathogenic Theileria orientalis Ikeda genotype from confined dairy cattle, in Hebei, China. Parasitol Res. 2022;121:395‐402. - PubMed
    1. Thompson AT, White S, Shaw D, et al. Theileria orientalis Ikeda in host‐seeking Haemaphysalis longicornis in Virginia, U.S.A. Ticks Tick Borne Dis. 2020;11:101450. - PubMed
    1. Eamens GJ, Bailey G, Jenkins C, Gonsalves JR. Significance of Theileria orientalis types in individual affected beef herds in New South Wales based on clinical, smear and PCR findings. Vet Parasitol. 2013;196(1–2):96‐105. - PubMed
    1. Ceci L, Iarussi F, Greco B, Lacinio R, Fornelli S, Carelli G. Retrospective study of hemoparasites in cattle in southern Italy by reverse line blot hybridization. J Vet Med Sci. 2014;76(6):869‐875. - PMC - PubMed

LinkOut - more resources