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. 2024 Oct 24;14(1):25118.
doi: 10.1038/s41598-024-75814-2.

Integrating an antimicrobial nanocomposite to bioactive electrospun fibers for improved wound dressing materials

Victoria Leonor Reyes-Guzmán  1 , Luis Jesús Villarreal-Gómez  2   3 , Rubi Vázquez-Mora  4 , Yesica Itzel Méndez-Ramírez  4 , Juan Antonio Paz-González  1 , Arturo Zizumbo-López  5 , Hugo Borbón  6 , Eder Germán Lizarraga-Medina  1 , José Manuel Cornejo-Bravo  7 , Graciela Lizeth Pérez-González  1 , Arturo Sinue Ontiveros-Zepeda  8 , Armando Pérez-Sánchez  1 , Elizabeth Chavira-Martínez  9 , Rafael Huirache-Acuña  10 , Yoxkin Estévez-Martínez  11
Affiliations

Integrating an antimicrobial nanocomposite to bioactive electrospun fibers for improved wound dressing materials

Victoria Leonor Reyes-Guzmán et al. Sci Rep. .

Abstract

This study investigates the fabrication and characterization of electrospun poly (ε-caprolactone)/poly (vinyl pyrrolidone) (PCL/PVP) fibers integrated with a nanocomposite of chitosan, silver nanocrystals, and graphene oxide (ChAgG), aimed at developing advanced wound dressing materials. The ChAgG nanocomposite, recognized for its antimicrobial and biocompatible properties, was incorporated into PCL/PVP fibers through electrospinning techniques. We assessed the resultant fibers' morphological, physicochemical, and mechanical properties, which exhibited significant enhancements in mechanical strength and demonstrated effective antimicrobial activity against common bacterial pathogens. The findings suggest that the PCL/PVP-ChAgG fibers maintain biocompatibility and facilitate controlled therapeutic delivery, positioning them as a promising solution for managing chronic and burn-related wounds. This study underscores the potential of these advanced materials to improve healing outcomes cost-effectively, particularly in settings plagued by high incidences of burn injuries. Further clinical investigations are recommended to explore these innovative fibers' full potential and real-world applicability.

Keywords: Chitosan; Electrospinning; Graphene oxide; Silver nanocrystal; Wound dressings.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Electrospun fibers in white and de air surrounded in red. Images were developed using software VGSTUDIO MAX (Version 2022.4) (https://www.volumegraphics.com/en/products/vgsm.html).
Figure 2
Figure 2
3D volume from X-ray tomography of PCL/PVP and PCL/PVP-ChAgG fibers. Analysis of interconnected pores. Images were developed using software VGSTUDIO MAX (Version 2022.4) (https://www.volumegraphics.com/en/products/vgsm.html).
Figure 3
Figure 3
Mechanical behavior of PCL/PVP and PCL/PVP-ChAgG fibers. (A) Stress-strain curves. (B) Elastic modulus. (C) Tensile strength. (D) Elongation at the break.
Figure 4
Figure 4
(A) Antibacterial study of PCL/PVP and PCL/PVP-ChAgG fibers against Staphylococcus aureus after 24, 48 and 72 h of exposition. Studies were made by triplicate. (B) Antibacterial study of PCL/PVP and PCL/PVP-ChAgG fibers against Escherichia coli after 24, 48 and 72 h of exposition. Studies were made by triplicate.
Figure 5
Figure 5
MTT cell viability test of PCL/PVP and PCL/PVP-ChAgG fibers after 24 h of exposition. Studies were made by triplicate.
See this image and copyright information in PMC

References

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