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. 2024 Oct 9:11:1463391.
doi: 10.3389/fmed.2024.1463391. eCollection 2024.

Profiling cytokines in peritoneal effluent through a targeted multiplex cytokine panel provides novel insight into the localized proinflammatory processes in patients undergoing peritoneal dialysis

Patrycja Okulewicz  1 , Bartosz Wojciuk  2 , Iwona Wojciechowska-Koszko  2 , Leszek Domański  1 , Edyta Gołembiewska  1
Affiliations

Profiling cytokines in peritoneal effluent through a targeted multiplex cytokine panel provides novel insight into the localized proinflammatory processes in patients undergoing peritoneal dialysis

Patrycja Okulewicz et al. Front Med (Lausanne). .

Abstract

Objectives: The number of relevant markers indicating local intraperitoneal inflammation in patients undergoing peritoneal dialysis (PD) is limited. Therefore, this study aimed to evaluate the compatibility of peritoneal effluent (PE) for proteomic analysis and assess its potential utility in immunoprofiling studies.

Methods: This pilot study included six PD patients from the Peritoneal Dialysis Center, Department of Nephrology, Transplantology, and Internal Medicine in Szczecin, Poland. All patients were clinically stable, with no signs of infections or malignancy at the time of study. PE samples were collected during routine surveillance visits at the Peritoneal Dialysis Center. Proteomic analysis of the samples was conducted using the Olink® (Olink Proteomics AB, Uppsala, Sweden) Target 48 Cytokine panel.

Results: PE samples were successfully analyzed, with 28 out of 45 proteins found within the limit of quantitation (LOQ) and 32 out of 45 proteins detected above the limit of detection (LOD). No significant interference from the matrix was observed in the assay. Biomarkers associated with low-grade inflammation showed varied levels, and the observed patterns were comparable across all patients.

Conclusion: This study suggests that utilizing a cytokine panel with relative quantification is a promising method for PE immunoprofiling.

Keywords: IL6; chronic kidney disease; effluent; inflammation; peritoneal dialysis; proteomics.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Detectability of 45 cytokines was measured using the Olink® Target 48 panel in peritoneal effluent of PD patients. Red bars indicate the percentage detectability for each assay (generated by Olink®).
Figure 2
Figure 2
Heatmap of cytokine concentrations among the patients. Low-grade and chronic inflammation markers of interest are highlighted in red (visualized using Microsoft Excel).
Figure 3
Figure 3
Relationships between IL6 and CCL11, CCL13, CCL19, CCL4, FLT3LG, and IL18 concentrations (visualized using Microsoft Excel).
See this image and copyright information in PMC

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