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. 2024 Dec:81:103589.
doi: 10.1016/j.scr.2024.103589. Epub 2024 Oct 19.

Generation of IPi002-A/B/C human induced pluripotent stem cell lines from MARCH amniotic fluid cells

Mikaël Boullé  1 , Ambre Leleu  2 , Siham Schacre  3 , Céline Banal  4 , Alix Boucharlat  3 , Solène Renault  2 , Marcel Hollenstein  5 , Patrick Frosk  6 , Frank Yates  2 , Nathalie Lefort  4 , Fabrice Agou  7
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Free article

Generation of IPi002-A/B/C human induced pluripotent stem cell lines from MARCH amniotic fluid cells

Mikaël Boullé et al. Stem Cell Res. 2024 Dec.
Free article

Abstract

Human induced pluripotent stem cells (hiPSCs) have become a revolutionary tool in biomedical research due to their unique in vitro properties and fate versatility. They offer insights into development or genetic disorders, facilitate drug discovery and hold promise for regenerative medicine. Here we generated three hiPSC cells - IPi002-A/B/C - from primary amniotic fluid cells (AFCs) obtained via amniocentesis for the prenatal diagnosis of MARCH syndrome: Multinucleated neurons, Anhydramnios, Renal dysplasia, Cerebellar hypoplasia, and Hydranencephaly. These AFCs underwent reprogramming through non-integrative viral transduction and the resulting hiPSCs exhibited normal karyotype and expressed typical pluripotency markers.

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Conflict of interest statement

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Fabrice Agou and Mikael Boulle reports financial support was provided by Finnish Academy of Science and Letters. Fabrice Agou reports financial support was provided by French National Research Agency. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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