Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct 14;10(10):658.
doi: 10.3390/gels10100658.

Development and Evaluation of the Acaricidal Activity of Xantan Gum-Based Hydrogel and Polymeric Nanoparticles Containing Achyrocline satureioides Extract

Rafaela Regina Fantatto  1 , Annelize Rodrigues Gomes  1 , João Vitor Carvalho Constantini  1 , Camila Fernanda Rodero  1 , Marlus Chorilli  1 , Ana Carolina de Souza Chagas  2 , Ana Melero  3 , Rosemeire Cristina Linhari Rodrigues Pietro  1
Affiliations

Development and Evaluation of the Acaricidal Activity of Xantan Gum-Based Hydrogel and Polymeric Nanoparticles Containing Achyrocline satureioides Extract

Rafaela Regina Fantatto et al. Gels. .

Abstract

The Rhipicephalus microplus tick causes enormous economic losses in livestock farming around the world. Despite several promising studies carried out with plant extracts such as Achyrocline satureioides against this ectoparasite, a major obstacle is related to pharmaceutical presentation forms. There is no study showing xantan gum-based hydrogel and polycaprolactone nanoparticles containing A. satureioides extract against R. microplus larvae. The objective of this study was to incorporate A. satureioides extract to develop a nanoformulation (AScn) and a hydrogel (ASlh) and evaluate them against R. microplus larvae with the purpose of increasing the contact time of the extract with the larvae and improve the effectiveness. The ethanolic extracts were incorporated in polycaprolactone nanoparticles and characterized via analysis of the mean hydrodinamic diameter and polidispersity index. The xanthan gum-based hydrogel formulation was prepared with crude extract of A. satureioides 40 mg/mL, 0.25% xanthan gum, and 8% poloxamer, to determine the bioadhesiveness of the formulation in bovine leather and the flow rate of the formulation in the animal. The results in larvae demonstrated that when evaluated in the form of a hydrogel (ASlh), mortality was higher, with 91.48% mortality at a concentration of 20 mg/mL presenting itself as an interesting alternative for controlling this ectoparasite.

Keywords: Achyrocline satureioides; Rhipicephalus microplus; acaricidal activity; hydrogel; xanthan gum.

PubMed Disclaimer

Conflict of interest statement

Ana Carolina de Souza Chagas was employed by EMBRAPA-Brazilian Agricultural Research Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Chromatogram obtained from A. satureioides (ASb) inflorescences. Analysis conditions: methanol (LC-MS grade) (MeOH): MilliQ water at 1% acetic acid with a gradient of 38:100% over 45 min, 100% MeOH over 10 min and 100:32% over 7 min and a flow of 0.5 mL/min.
Figure 2
Figure 2
Quercetin, 3-O-methylquercetin, and kaempferol molecules found in the ethanolic extract of A. satureioides (ChemDraw Ultra 12.0).
Figure 3
Figure 3
(a) Strength of bioadhesion of samples on bovine leather. (b) Bioadhesion work of the samples on the leather.
Figure 4
Figure 4
Flow distance for hydrogel formulation containing extract (ASlh), blank (xanthan gum and poloxamer), and water in contact with the bovine leather.
Figure 5
Figure 5
Test to determine the flow of formulations on bovine leather (Rafaela Fantatto).
See this image and copyright information in PMC

References

    1. Calvano M.P.C.A., Brumatti R.C., Barros J.C., Garcia M.V., Martins K.R., Andreotti R. Bioeconomic simulation of Rhipicephalus microplus infestation in different beef cattle production systems in the Brazilian Cerrado. Agric. Syst. 2021;194:103247. doi: 10.1016/j.agsy.2021.103247. - DOI
    1. Rodriguez-Vivas R.I., Jonsson N.N., Bhushan C. Strategies for the control of Rhipicephalus microplus ticks in a world of conventional acaricide and macrocyclic lactone resistance. Parasitol. Res. 2018;117:3–29. doi: 10.1007/s00436-017-5677-6. - DOI - PMC - PubMed
    1. Chagas A.C.S., Domingues L.F., Fantatto R.R., Giglioti R., Oliveira M.C.S., Oliveira D.H., Mano R.A., Jacob R.G. In vitro and in vivo acaricide action of juvenoid analogs produced from the chemical modification of Cymbopogon spp. and Corymbia citriodora essential oil on the cattle tick Rhipicephalus (Boophilus) microplus. Vet. Parasitol. 2014;205:277–284. doi: 10.1016/j.vetpar.2014.06.030. - DOI - PubMed
    1. Sousa A., Castro G.S., Tavares C., Vale T.L., Costa-Júnior L., Soares A. In Vitro Assessment of the Acaricidal Activity of a Carvacrol Shampoo on Tick Larvae. Exp. Parasitol. 2022;242:108364. doi: 10.1016/j.exppara.2022.108364. - DOI - PubMed
    1. Camargo B.A.F., Silva D.E.S., Silva A.N., Campos D.L., Ribeiro T.R.M., Mieli M.J., Zanatta M.B.T., Silva P.B., Pavan F.R., Moreira C.G., et al. A new silver (I) coordination compound loaded into polymeric nanoparticles as a strategy to improve in vitro anti-Helicobacter pylori activity. Mol. Pharm. 2020;17:2287–2298. doi: 10.1021/acs.molpharmaceut.9b01264. - DOI - PubMed

LinkOut - more resources