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. 2024 Oct 15;15(10):306.
doi: 10.3390/jfb15100306.

Early Effects of Porcine Placental Extracts and Stem Cell-Derived Exosomes on Aging Stress in Skin Cells

Takaaki Matsuoka  1 , Katsuaki Dan  2   3 , Keita Takanashi  2 , Akihiro Ogino  4
Affiliations

Early Effects of Porcine Placental Extracts and Stem Cell-Derived Exosomes on Aging Stress in Skin Cells

Takaaki Matsuoka et al. J Funct Biomater. .

Abstract

The initial efficacy of placental extracts (Pla-Exts) and human mesenchymal stem-cell-derived exosomes (hMSC-Exo) against aging-induced stress in human dermal fibroblasts (HDFs) was examined. The effect of Pla-Ext alone, hMSC-Exo alone, the combined effect of Pla-Ext and hMSC-Exo, and the effect of hMSC-Exo (Pla/MSC-Exo) recovered from cultures with Pla-Ext added to hMSC were verified using collagen, elastin, and hyaluronic acid synthase mRNA levels for each effect. Cells were subjected to photoaging (UV radiation), glycation (glycation end-product stimulation), and oxidation (H2O2 stimulation) as HDF stressors. Pla-Ext did not significantly affect normal skin fibroblasts with respect to intracellular parameters; however, a pro-proliferative effect was observed. Pla-Ext induced resistance to several stresses in skin fibroblasts (UV irradiation, glycation stimulation, H2O2 stimulation) and inhibited reactive oxygen species accumulation following H2O2 stimulation. Although the effects of hMSC-Exo alone or the combination of hMSC-Exo and Pla-Ext are unknown, pretreated hMSC-Exo stimulated with Pla-Ext showed changes that conferred resistance to aging stress. This suggests that Pla-Ext supplementation may cause some changes in the surface molecules or hMSC-Exo content (e.g., microRNA). In skin cells, the direct action of Pla-Ext and exosomes secreted from cultured hMSCs pretreated with Pla-Ext (Pla/MSC-Exo) also conferred resistance to early aging stress.

Keywords: aging; human mesenchymal stem-cell-derived exosomes; placental extracts; senescence.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Effects of Pla-Ext on (a) collagen, (b) elastin, and (c) hyaluronic acid synthase mRNA expression levels in normal human dermal fibroblasts. Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; placenta extract-stimulated human mesenchymal stem-cell-derived exosomes.
Figure 2
Figure 2
Effects of Pla-Ext on normal human dermal fibroblast proliferation. Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; placenta extract-stimulated human mesenchymal stem-cell-derived exosomes. * p < 0.05 vs. control (DW).
Figure 3
Figure 3
Effects of Pla-Ext on (a) collagen, (b) elastin, and (c) hyaluronic acid synthase mRNA expression levels in UV-treated human dermal fibroblasts (5 or 25 min). Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; placenta extract-stimulated human mesenchymal stem-cell-derived exosomes. * p < 0.001 vs. UV alone.
Figure 4
Figure 4
Effects of Pla-Ext on (a) collagen, (b) elastin, and (c) hyaluronic acid synthase mRNA expression levels in human dermal fibroblasts treated with advanced glycation end-products (AGE). Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; Placenta extract-stimulated human mesenchymal stem-cell-derived exosomes. * p < 0.001 vs. AGE alone.
Figure 5
Figure 5
Effects of Pla-Ext on (a) collagen, (b) elastin, and (c) hyaluronic acid synthase in human dermal fibroblasts treated with H2O2. Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; Placenta extract-stimulated human mesenchymal stem-cell-derived exosomes. * p < 0.001 vs. H2O2 alone.
Figure 6
Figure 6
Effects of Pla-Ext on the generation of reactive oxygen species (ROS) in human dermal fibroblasts treated with H2O2. Pla-Ext, placental extract; hMSC-Exo, human mesenchymal stem-cell-derived exosomes; Pla extract-stimulated hMSC-Exo; placenta extract-stimulated human mesenchymal stem-cell-derived exosomes. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. H2O2 alone.
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References

    1. Shin S.H., Lee Y.H., Rho N.K., Park K.Y. Skin aging from mechanisms to interventions: Focusing on dermal aging. Front. Physiol. 2023;14:1195272. doi: 10.3389/fphys.2023.1195272. - DOI - PMC - PubMed
    1. Wang Y., Branicky R., Noë A., Hekimi S. Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J. Cell Biol. 2018;217:1915–1928. doi: 10.1083/jcb.201708007. - DOI - PMC - PubMed
    1. Lv J., Yang S., Lv M., Lv J., Sui Y., Guo S. Protective roles of mesenchymal stem cells on skin photoaging: A narrative review. Tissue Cell. 2022;76:101746. doi: 10.1016/j.tice.2022.101746. - DOI - PubMed
    1. Chen B., Sun Y., Zhang J., Zhu Q., Yang Y., Niu X., Deng Z., Li Q., Wang Y. Human embryonic stem cell-derived exosomes promote pressure ulcer healing in aged mice by rejuvenating senescent endothelial cells. Stem Cell Res. Ther. 2019;10:142. doi: 10.1186/s13287-019-1253-6. - DOI - PMC - PubMed
    1. Yang A., Shuyan L., Xiaojie T., Shiou Z., Fangfei N., Yonghuan Z., Luosha G., Chunlei Z., Baicheng W., Wei W., et al. Exosomes from adipose-derived stem cells and application to skin wound healing. Cell. Prolif. 2021;54:e12993. doi: 10.1111/cpr.12993. - DOI - PMC - PubMed

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