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. 2024 Sep 24;13(10):822.
doi: 10.3390/pathogens13100822.

Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC) from Southern Alberta, Canada, from 2020 to 2022

Heather Glassman  1 , Vivien Suttorp  2 , Theron White  3 , Kim Ziebell  4 , Ashley Kearney  5 , Kyrylo Bessonov  4 , Vincent Li  6 , Linda Chui  1   6
Affiliations

Clinical Outcomes and Virulence Factors of Shiga Toxin-Producing Escherichia coli (STEC) from Southern Alberta, Canada, from 2020 to 2022

Heather Glassman et al. Pathogens. .

Abstract

Shiga toxin-producing Escherichia coli (STEC) can cause severe clinical disease in humans, particularly in young children. Recent advances have led to greater availability of sequencing technologies. We sought to use whole genome sequencing data to identify the presence or absence of known virulence factors in all clinical isolates submitted to our laboratory from Southern Alberta dated 2020-2022 and correlate these virulence factors with clinical outcomes obtained through chart review. Overall, the majority of HUS and hospitalizations were seen in patients with O157:H7 serotypes, and HUS cases were primarily in young children. The frequency of virulence factors differed between O157:H7 and non-O157 serotypes. Within the O157:H7 cases, certain virulence factors, including espP, espX1, and katP, were more frequent in HUS cases. The number of samples was too low to determine statistical significance.

Keywords: Shiga toxin-producing Escherichia coli; virulence factors; whole genome sequencing.

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Conflict of interest statement

The authors declare no conflicts of interest.

References

    1. Bruyand M., Mariani-Kurkdjian P., Gouali M., De Valk H., King L.A., Le Hello S., Bonacorsi S., Loirat C. Hemolytic uremic syndrome due to Shiga toxin-producing Escherichia coli infection. Med. Mal. Infect. 2018;48:167–174. doi: 10.1016/j.medmal.2017.09.012. - DOI - PubMed
    1. Persson S., Olsen K.E.P., Ethelberg S., Scheutz F. Subtyping method for Escherichia coli Shiga toxin (Verocytotoxin) 2 variants and correlations to clinical manifestations. J. Clin. Microbiol. 2007;45:2020–2024. doi: 10.1128/JCM.02591-06. - DOI - PMC - PubMed
    1. Karmali M.A. Host and pathogen determinants of verocytotoxin-producing Escherichia coli-associated hemolytic uremic syndrome. Kidney Int. 2009;75:S4–S7. doi: 10.1038/ki.2008.608. - DOI - PubMed
    1. Bessonov K., Laing C., Robertson J., Yong I., Ziebell K., Gannon V.P.J., Nichani A., Arya G., Nash J.H.E., Christianson S. ECTyper: In silico Escherichia coli serotype and species prediction from raw and assembled whole-genome sequence data. Microb. Genom. 2021;7:000728. doi: 10.1099/mgen.0.000728. - DOI - PMC - PubMed
    1. Chen S. Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp. iMeta. 2023;2:e107. doi: 10.1002/imt2.107. - DOI - PMC - PubMed

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