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. 2024 Sep 28;14(10):526.
doi: 10.3390/metabo14100526.

Comparative Metabolic Profiling in Drosophila suzukii by Combined Treatment of Fumigant Phosphine and Low Temperature

Junbeom Lee  1 , Hyun-Kyung Kim  2   3 , Jong-Chan Jeon  2 , Seung-Ju Seok  2 , Gil-Hah Kim  2 , Hyun-Na Koo  2 , Dae-Weon Lee  1   3
Affiliations

Comparative Metabolic Profiling in Drosophila suzukii by Combined Treatment of Fumigant Phosphine and Low Temperature

Junbeom Lee et al. Metabolites. .

Abstract

Background/Objectives: The mechanisms of action of phosphine are diverse and include neurotoxicity, metabolic inhibition, and oxidative stress; however, its efficacy at low temperatures is unclear. Methods: Comparative metabolomics is suitable for investigating the response of the spotted-wing fly Drosophila suzukii to exposure toward a combination of cold stimuli and fumigant PH3. Results: Under this combined exposure, 52 metabolites exhibiting significant differences in stress were identified and their physiological roles were analyzed in the Drosophila metabolic pathway. Most metabolites were involved in amino acids, TCA cycle, and nucleic acids. In addition, the alteration levels of cell membrane lipids, such as glycerophospholipids, sphingolipids, and glycerolipids, clearly showed changes in the combined treatment compared to PH3 and low temperatures alone. Aconitic acid, a component of the TCA cycle, was completely inhibited by the combined treatment. Conclusions: These results suggest that treatment-specific indicators could be useful biomarkers to indicate the synergistic effects of PH3 and low temperature on energy metabolism.

Keywords: Drosophila suzukii; lipidomics; low temperature; metabolomics; phosphine.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Enrichment ratio and pathway impact scores. Metabolite set enrichment analysis in altered metabolites. (A) Low temperature, (B) PH3, and (C) combined treatment. Analysis was performed using the Kyoto Encyclopedia of Genes and Genomes database.
Figure 2
Figure 2
Lipidomic profiling altered by exposure to low temperature and PH3. (A) The number of lipids showing relative increases and decreases in D. suzukii after stress. (B) Heatmap of membrane-associated lipids. FA: fatty acid; GL: glycerolipid; GP: glycerophospholipid; PK: polyketide; PR: prenol lipid; SP: sphingolipid; ST: sterol lipid.
Figure 3
Figure 3
Top 20 signaling pathways enriched at low temperature, PH3, and combined treatment. Numbers and colors indicate the ranking (high: green; low: yellow) of the respective signaling pathways.
See this image and copyright information in PMC

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