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. 2024 Oct 11;11(10):494.
doi: 10.3390/vetsci11100494.

Effect of Three Commercially Available Extenders Containing Phospholipids of Different Sources on Skopelos Buck Liquid-Stored Sperm Quality

Aikaterini Saratsi  1 , Foteini Samartzi  1 , Dimitrios Tsiokos  2 , Ekaterini K Theodosiadou  3 , Ioannis Panagiotidis  4 , Christina Ligda  1 , Constantinos A Rekkas  1
Affiliations

Effect of Three Commercially Available Extenders Containing Phospholipids of Different Sources on Skopelos Buck Liquid-Stored Sperm Quality

Aikaterini Saratsi et al. Vet Sci. .

Abstract

The effect of four extenders on buck semen quality parameters was examined during a 48 h liquid storage. Semen was collected from six Skopelos bucks and diluted in the following extenders, containing: soy lecithin (SL, OviXcell®), plant phospholipids (PP, AndroMed®), egg yolk lecithin (EY, Steridyl®), or no phospholipids (basic extender). Samples were stored at 5 °C for 48 h and assessed at 0, 24 and 48 h for viability (eosin-nigrosin), acrosome integrity (SpermBlue®), membrane functional integrity (HOST), mitochondrial function (Rhodamine 123/SYBR-14/PI) and motility parameters (CASA). No significant reduction in total or progressive spermatozoa motility and mitochondrial function was observed at 24 h, whereas they all dropped significantly at 48 h, in all extenders. Spermatozoa viability, cell membrane functionality and acrosome integrity dropped progressively (0 h > 24 h > 48 h) in all groups. No significant difference among extenders was observed concerning spermatozoa mitochondrial function. Overall, spermatozoa viability, cell membrane functionality and acrosome integrity were higher in the three commercial extenders, compared to the basic extender. SL and EY extenders (OviXcell® and Steridyl®, respectively) preserved viability more effectively than the PP extender (AndroMed®). Total motility was higher in the PP extender, compared to the SL extender. Spermatozoa acrosome integrity tended to be higher in the EY extender compared to all the other extenders. Further investigation of the protective potential of different types of cryoprotectants on liquid buck semen storage is important.

Keywords: buck semen; egg yolk; extender; liquid storage; plant phospholipids; soy lecithin.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Percentage (mean ± SE) of live spermatozoa measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage (p is provided for comparisons between extenders within time and between time within extenders).
Figure 2
Figure 2
Percentage (mean ± SE) of spermatozoa with a functional cell membrane measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage p is provided for comparisons between extenders within time and between time within extenders).
Figure 3
Figure 3
Percentage (mean ± SE) of spermatozoa with an intact acrosome membrane measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage (p is provided for comparisons between extenders within time and between time within extenders).
Figure 4
Figure 4
Percentage (mean ± SE) of live spermatozoa with high mitochondrial membrane potential measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage (p is provided for comparisons between extenders within time and between time within extenders).
Figure 5
Figure 5
Percentage (mean ± SE) of total spermatozoa motility measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage (p is provided for comparisons between extenders within time and between time within extenders).
Figure 6
Figure 6
Percentage (mean ± SE) of spermatozoa progressive motility measured in Skopelos buck semen diluted with extenders containing soy lecithin (OviΧcell®), plant phospholipids (AndroMed®), egg yolk lecithin (Steridyl®) or no phospholipids (basic extender) after 0, 24 and 48 h of liquid storage (p is provided for comparisons between extenders within time and between time within extenders).
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