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. 2024 Oct 25;19(10):e0310453.
doi: 10.1371/journal.pone.0310453. eCollection 2024.

Recovery and partial isolation of ⍺-mangostin from mangosteen pericarpsvia sequential extraction and precipitation

Moh Moh Han  1 , Preuk Tangpromphan  2 , Amaraporn Kaewchada  3 , Attasak Jaree  2
Affiliations

Recovery and partial isolation of ⍺-mangostin from mangosteen pericarpsvia sequential extraction and precipitation

Moh Moh Han et al. PLoS One. .

Abstract

This study introduced an innovative sequential extraction methodology designed for the efficient recovery of alpha-mangostin (⍺-M) from mangosteen pericarps. Alpha-mangostin, renowned for its pharmacological properties including anti-inflammatory, anti-cancer, and anti-bacterial effects, has garnered significant attention across diverse industries. The proposed method of sequential extraction achieved 73% recovery and a yield of 46.75 mg/g based on the weight/weight percentage of the mass of ⍺-M extracted from the sequence and the mass of raw material. Furthermore, the purity of the dried product was 67.9%. The sequence solvent extraction system, comprising water, hexane, and acetonitrile, plays a pivotal role in enhancing the efficacy of the extraction process. Notably, this methodology offers a cost-effective alternative to conventional extraction methods. It reduces the need for complex equipment and processes, positioning it as a resource-efficient extraction technique in comparison to existing methodologies. This novel sequential extraction method presents a promising avenue for the economical and sustainable recovery of alpha-mangostin (⍺-M) from pericarps.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Crosscurrent extraction.
In this experiment, the substrate powder (S) was mixed with solvent (E). The mass fraction of ⍺-M in the powder and in the solution is designated as x and y, respectively. The mangosteen pericarp powder was placed in a glass tube to be extracted. At the bottom of the column, there was a valve (initially closed) that was used to collect liquid from the column by gravity. The amount of powder in the 1st stage is denoted as S0. Then 50 mL of solvent (Acetonitrile), E1,0, was poured into the column, providing the solid and solvent ratio of 1 g: 8.24 mL. The solvent passed through the column retention time for 10 mins at room temperature with the percolation method for each batch extraction. The solvent extract E1, was removed by pipetting out with a syringe, and filter, and analyzed that extract with HPLC. The solid S1 was used as feed for the 2nd extraction and the same amount of solvent E2,0 was added. The process was repeated for 8 cycles. The mass fraction was calculated using Eq (1).
Fig 2
Fig 2. Sequential extraction method.
Fig 3
Fig 3. Chromatogram of (a) standard of ⍺-M (b) extract solution obtained from 1st -step percolation using acetonitrile as solvent.
Fig 4
Fig 4. Amounts of ⍺-M obtained from multi-stage percolation.
Fig 5
Fig 5. Solvent distribution factor of acetonitrile, hexane, and water.
Fig 6
Fig 6. Chromatogram of extract solution obtained from different stages of sequential extraction (a) water (b) water-hexane (c) water-hexane-acetonitrile.
Fig 7
Fig 7. Recovery of crude extract via precipitation.
See this image and copyright information in PMC

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