Effects of Supplementation of Different Antioxidants to Cryopreservation Extender on the Post-Thaw Quality of Rooster Semen-A Meta-Analysis

Abstract

The standardization of the semen cryopreservation technique could be an effective tool in poultry for the conservation of genetic resources. During this process, the production of reactive oxygen species increases, leading to oxidative stress that causes damage to the spermatozoa. To reduce this effect, the addition of exogenous antioxidants in the cryopreservation diluent has been reported to be effective. Multiple antioxidants such as catalase, vitamin E, cysteamine, ergothioneine, and serine have been studied in roosters. Therefore, the present investigation aims to perform a meta-analysis to determine if the use of the aforementioned antioxidants added to the cryopreservation extender produces an improvement in semen quality parameters in roosters after thawing. After collecting the data, a discriminant canonical analysis was performed to determine which of the selected semen quality traits provided the most information, with hypo-osmotic swelling test (HOST), viability, and total motility variables showing the highest discriminatory power. However, according to the descriptive statistics, catalase and serine are the antioxidants that improve a greater number of seminal quality parameters, and since catalase gives the most favorable results for most of the discriminating variables, it could be the antioxidant of choice.

Keywords: antioxidants; cryopreservation; rooster; semen.

Figure 1

Canonical variable functions and percentages of self-explained and cumulative variance. The eigenvalue of each discriminant function is used to measure each function’s discriminative power.

Figure 2

Correlation matrix between the different semen quality parameters studied. TM: total motility; PM: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; LIN: linearity; STR: straightness; HOST: hypo-osmotic swelling test.

Figure 3

Standardized canonical discriminant function coefficients. TM: total motility; PM: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; LIN: linearity; STR: straightness; HOST: hypo-osmotic swelling test.

Figure 4

Territorial map depicting the results of the canonical discriminant analysis on the different antioxidants studied. The larger the differences between each point, the better the predictive power of the DCA classifying them.