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. 2024 Oct 18;16(20):3529.
doi: 10.3390/nu16203529.

Subacute Effects of Moderate-Intensity Aerobic Exercise in the Fasted State on Cell Metabolism and Signaling in Sedentary Rats

Layane Ramos Ayres  1   2 , Éverton Lopes Vogt  3 , Helena Trevisan Schroeder  1 , Mariana Kras Borges Russo  1 , Maiza Cristina Von Dentz  3 , Débora Santos Rocha  3 , Jorge Felipe Argenta Model  3 , Lucas Stahlhöfer Kowalewski  1 , Samir Khal de Souza  3 , Vitória de Oliveira Girelli  3 , Jerônimo da Rosa Coelho  1   2 , Nathalia de Souza Vargas  1   2 , Alvaro Reischak-Oliveira  2 , Paulo Ivo Homem de Bittencourt Jr  1 , Eurico Nestor Wilhelm  4 , Anapaula Sommer Vinagre  3 , Mauricio Krause  1   2
Affiliations

Subacute Effects of Moderate-Intensity Aerobic Exercise in the Fasted State on Cell Metabolism and Signaling in Sedentary Rats

Layane Ramos Ayres et al. Nutrients. .

Abstract

Background: Physical inactivity induces insulin resistance (IR) and metabolic imbalances before any significant changes in adiposity. Recent studies suggest that the beneficial effects of exercise can be potentiated if performed while fasting. This work aimed to compare the subacute effects of fed- and fasted-state single-bout exercise on biochemical parameters and cellular signaling in the metabolism. Methods: The animals were allocated into fed rest (FER), fasting rest (FAR), fed exercise (FEE), and fasting exercise (FAE) groups. The exercise protocol was a 30 min treadmill session at 60% of V˙O2max. The fasting groups fasted for 8 h before exercise and were killed after 12 h post-exercise. Results: Soleus glycogen concentration increased only in the fasting groups, whereas the triglyceride (TGL) content increased in brown adipose tissue (BAT) and liver in the FAE. The FAE showed decreased plasma total cholesterol concentration compared withthe FAR group. Immunocontent of HSP70, SIRT1, UCP-1, and PGC1-α did not change in any tissue investigated. Conclusions: Our results indicate that physical exercise while fasting can have beneficial metabolic effects on sedentary animals. Remarkably, in the FAE group, there was a reduction in total plasma cholesterol and an increase in the capacity of BAT to metabolize and store nutrients in the form of TGLs.

Keywords: exercise; fasting; heat shock protein; sedentary behavior.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Experimental design. Groups: fed rest (FER), fed exercise (FEE), fasted rest (FAR), and fasted exercise (FAE).
Figure 2
Figure 2
Blood and tissue metabolism. Concentrationsof plasma glucose (A), TGL (B), total cholesterol (C), total proteins (D), and lactate (E) are presented.Groups: fed rest (FER), fed exercise (FEE), fasted rest (FAR), and fasted exercise (FAE). Data expressed as mean ± SD. * represents differences between groups. Letters represent significant differences between each experimental group.
Figure 3
Figure 3
Glycogen concentration in the tissues in the final experimental protocol. Gastrocnemius muscle (A), soleus muscle (B), liver (C), brown adipose tissue (D), and heart (E) glycogen concentrations are presented. Data expressed as mean ± SD. * represents differences between groups.
Figure 4
Figure 4
Concentration of triglycerides in the tissues in the end experimental protocol. Gastrocnemius muscle (A), soleus muscle (B), liver (C), brown adipose tissue (D), heart (E). Data expressed as mean ± SD. * represents differences between groups. Letters represent significant differences between each experimental group.
Figure 5
Figure 5
Protein immunocontent at 12 h post-experimental treatment. SIRT1 and HSP70 in the soleus (A,B) and gastrocnemius muscle (C,D), respectively. Data expressed as mean ± SD.
Figure 6
Figure 6
Protein immunocontent at 12 h post-experimental treatment. SIRT1(A) and HSP70 (B) in the liver. Data expressed as mean ± SD.
Figure 7
Figure 7
Protein immunocontent at 12 h post-experimental treatment. SIRT1 (A), HSP70 (B), UCP1 (C), PGC-1α (D) in BAT. Data expressed as mean ± SD.
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