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. 2025 Mar;22(3):e202402184.
doi: 10.1002/cbdv.202402184. Epub 2024 Nov 14.

Assessment of Artemisia Campestris L. Leaf Extract Effects on Polycystic Ovarian Syndrome in Rats, Antioxidant and α-Amylase Inhibition Activities

Inasse Cherfi  1   2 , Nasma Mahboub  1   2 , Ikram Toumi  1   2 , Salah Eddine Laouini  3   4 , Gamil Gamal Hasan  3 , Abderrhmane Bouafia  3   4 , Fahad Alharthi  5 , Talha Bin Emran  6   7   8
Affiliations

Assessment of Artemisia Campestris L. Leaf Extract Effects on Polycystic Ovarian Syndrome in Rats, Antioxidant and α-Amylase Inhibition Activities

Inasse Cherfi et al. Chem Biodivers. 2025 Mar.

Abstract

Polycystic Ovarian Syndrome (PCOS) is characterized by metabolic and reproductive dysfunction, often associated with elevated oxidative stress markers in the bloodstream. This study examines the potential antioxidant properties and α-amylase inhibitory activity of Artemisia campestris leaves extract (Artemisia campestris L) and its effects on rats with induced PCOS. Estradiol valerate was administered to ten mature Wistar rats to induce PCOS, while a control group consisted of five mature Wistar rats. Following a 16-day induction period, the rats were categorized into three groups: a control group, a PCOS group, and an experimental group receiving 200 mg/kg body weight of A. campestris L. extract orally for 15 days. Serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using the ELISA technique. The group treated with A. campestris L. extract exhibited significantly reduced LH levels compared to the PCOS group. Histomorphometric analysis indicated notable changes in follicle counts and the thickness of the theca layer. These findings suggest a significant alleviation of PCOS symptoms, potentially linked to the effects of A. campestris L. on oxidative stress pathways. Furthermore, aqueous extracts of A. campestris L. displayed potent in vitro inhibition of α-amylase, with an IC50 value of 2.418 μg/mL.

Keywords: Alpha amylase enzyme inhibition; Artemisia campestris L; Estradiol valesrate; Polycystic ovary syndrome.

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