doi: 10.1016/0006-291x(86)90543-7.
Guanine nucleotide-induced positive cooperativity in muscarinic-cholinergic antagonist binding
- PMID: 3947324
- DOI: 10.1016/0006-291x(86)90543-7
Item in Clipboard
Guanine nucleotide-induced positive cooperativity in muscarinic-cholinergic antagonist binding
Biochem Biophys Res Commun.
.
Abstract
The effect of guanine nucleotides on the binding of [3H] Quinuclidinyl benzilate to heart muscarinic receptors was studied. It was observed that GTP and Gpp (NH)p increased antagonist binding. Scatchard transformation of the data resulted in curvilinear plots (downward concavity) consistent with positive cooperativity. Graphic analysis for cooperativity indicated that guanine nucleotides: a) increased slightly but consistently the affinity for the antagonist (independent of receptor occupancy) and b) induce positive cooperativity in the binding of [3H]QNB (dependent of receptor occupancy).
Similar articles
-
Modulation of muscarinic cholinergic receptor affinity for antagonists in rat heart.J Pharmacol Exp Ther. 1984 Aug;230(2):424-30. J Pharmacol Exp Ther. 1984. PMID: 6379149
-
MgCl2-sensitive and Gpp(NH)p-sensitive antagonist binding states of rat heart muscarinic receptors: preferential detection at ambient temperature assay and location in two subcellular fractions.Mol Cell Biochem. 1990 May 10;94(2):133-46. doi: 10.1007/BF00214120. Mol Cell Biochem. 1990. PMID: 2374547
-
The biphasic response of muscarinic cholinergic receptors in cultured heart cells to agonists. Effects on receptor number and affinity in intact cells and homogenates.J Biol Chem. 1982 Sep 10;257(17):10344-56. J Biol Chem. 1982. PMID: 7107608
-
Differential regulation of putative M1/M2 muscarinic receptors: implications for different receptor-effector coupling mechanisms.Adv Exp Med Biol. 1984;175:99-114. doi: 10.1007/978-1-4684-4805-4_8. Adv Exp Med Biol. 1984. PMID: 6208760 Review. No abstract available.
-
Positron emission tomography and cholinergic mechanisms: an overview.Prog Brain Res. 1990;84:347-59. doi: 10.1016/s0079-6123(08)60918-8. Prog Brain Res. 1990. PMID: 2267307 Review. No abstract available.
Cited by
-
Oligomerization of G protein-coupled receptors: past, present, and future.Biochemistry. 2004 Dec 21;43(50):15643-56. doi: 10.1021/bi047907k. Biochemistry. 2004. PMID: 15595821 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
