Artificial Spidroin Nanogenerator-Based Articulus Wound Dressing

Abstract

Articulus wound infection is a threat to human health. Existing medical materials have poor biocompatibility and may contain harmful chemicals, causing allergies and secondary infections. Therefore, there is an urgent need to develop innovative medical materials. Materials made of artificial spider silk proteins have been widely used in wound healing because of their good biocompatibility, biodegradability, cell adhesion and bioelectronic properties.

Keywords: Artificial Spidroin; Nanogenerator; Wound Dressing.

Figure 1

a) Schematic diagrams of the molecular structure of wild‐type spiders (2REP) and mutant spiders (2REPM) and their self‐assembled spiders. b) Expression of chimeric spidroins 2REP and 2REPM with tyrosine mutations (MW: 33.2 kDa). c) Relationship between the binding free energy of the 2REP dimer and 2REPM dimer and the change in time. The number of hydrogen bonds between 2REP/2REPM dimers and between 2REP/2REPM dimers and aqueous solvents. Reprinted with permission from Ref. [21] copyright 2024 American Chemical Society. d) To reduce the difficulty of expression and enhance the mechanical properties of the protein, the characteristic polyalanine motif was replaced by an amyloid polypeptide sequence with a cross β‐spine structure. e) Solid powder of artificial spidroin. f) SDS−PAGE analysis of artificial spidroins. Note: 4rep (primarily artificial spidroin, 40.4 kDa); Cs‐6rep (artificial spidroin with an increased number of microcrystalline regions, 39.8 kDa); Amy‐6rep (artificial spidroin with increased microcrystalline regions and amyloid polypeptide replacement (39.6 kDa)). (i) Protein purification diagram of Amy‐6rep (ii). Reprinted with permission from ref., copyright 2024 Wiley‐VCH GmbH.

Figure 2

a) A nanofriction generator was prepared on the basis of artificial nanospider silk with tribopower performance via high‐pressure electrospinning technology. b) SEM image of nanospidroin fibers obtained by high‐pressure electrospinning. c) The potential generated by the friction of the nanospider fiber membrane was recorded by an oscilloscope. d) Charging of a 50 nF capacitor with nanospecidroin fibers under different rubbing frequencies. Reprinted with permission from ref., copyright 2024 Wiley‐VCH GmbH. e) KSM‐TENG structure based on spider/PU‐microneedle scaffold (SP‐MNs) film, polytetrafluoroethylene (PTFE) and Cu. f) Schematic diagram of the working principle of the KSM‐TENG under the vertical contact‐separation mode. Reprinted with permission from ref., copyright 2024 Elsevier.