Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun 24:9:335.
doi: 10.12688/wellcomeopenres.22469.1. eCollection 2024.

The genome sequence of a bluebottle fly, Calliphora vicina (Linnaeus, 1758)

Olga Sivell  1 ; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics team; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of a bluebottle fly, Calliphora vicina (Linnaeus, 1758)

Olga Sivell et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female Calliphora vicina (bluebottle blow fly; Arthropoda; Insecta; Diptera; Calliphoridae). The genome sequence is 706.5 megabases in span. Most of the assembly is scaffolded into 6 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 16.72 kilobases in length. Gene annotation of this assembly on Ensembl identified 13,436 protein coding genes.

Keywords: Calliphora vicina; Diptera; bluebottle blow fly; chromosomal; genome sequence.

PubMed Disclaimer

Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Photograph of the Calliphora vicina (NHMUK014111059; idCalVici1) specimen used for genome sequencing.
Figure 2.
Figure 2.. Genome assembly of Calliphora vicina, idCalVici1.1: metrics.
The BlobToolKit snail plot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 706,541,155 bp assembly. The distribution of sequence lengths is shown in dark grey with the plot radius scaled to the longest sequence present in the assembly (172,799,725 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 sequence lengths (131,702,511 and 114,622,126 bp), respectively. The pale grey spiral shows the cumulative sequence count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the diptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Calliphora%20vicina/dataset/idCalVici1_1/snail.
Figure 3.
Figure 3.. Genome assembly of Calliphora vicina, idCalVici1.1: BlobToolKit GC-coverage plot.
Sequences are coloured by phylum. Circles are sized in proportion to sequence length. Histograms show the distribution of sequence length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Calliphora vicina/dataset/idCalVici1_1/blob.
Figure 4.
Figure 4.. Genome assembly of Calliphora vicina, idCalVici1.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all sequences. Coloured lines show cumulative lengths of sequences assigned to each phylum using the buscogenes taxrule in the BlobToolKit environment. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Calliphora%20vicina/dataset/idCalVici1_1/cumulative.
Figure 5.
Figure 5.. Genome assembly of Calliphora vicina, idCalVici1.1: Hi-C contact map of the idCalVici1.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=JYSjdNSRRIC0XJ3z8-tzbg.
See this image and copyright information in PMC

Similar articles

  • The genome sequence of a bluebottle, Calliphora vomitoria (Linnaeus, 1758).
    Sivell O; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium. Sivell O, et al. Wellcome Open Res. 2023 Feb 21;8:93. doi: 10.12688/wellcomeopenres.18891.1. eCollection 2023. Wellcome Open Res. 2023. PMID: 38173563 Free PMC article.
  • The genome sequence of a conopid fly, Myopa testacea (Linnaeus, 1767).
    Falk S, Poole O; University of Oxford and Wytham Woods Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics team; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium. Falk S, et al. Wellcome Open Res. 2024 Feb 29;9:99. doi: 10.12688/wellcomeopenres.20647.1. eCollection 2024. Wellcome Open Res. 2024. PMID: 38689759 Free PMC article.
  • The genome sequence of a woodlouse fly, Melanophora roralis (Linnaeus, 1758).
    Mitchell R, Sivell O; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics team; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium. Mitchell R, et al. Wellcome Open Res. 2024 Oct 30;9:637. doi: 10.12688/wellcomeopenres.23295.1. eCollection 2024. Wellcome Open Res. 2024. PMID: 39659996 Free PMC article.
  • The genome sequence of the dark-edged bee fly, Bombylius major (Linnaeus, 1758).
    Lawniczak MKN, Crowley LM, McAlister E; University of Oxford and Wytham Woods Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium. Lawniczak MKN, et al. Wellcome Open Res. 2023 Sep 1;8:379. doi: 10.12688/wellcomeopenres.19804.1. eCollection 2023. Wellcome Open Res. 2023. PMID: 38533437 Free PMC article.
  • The genome sequence of a tachinid fly, Nowickia ferox (Panzer, 1809).
    Falk S, Raper C; University of Oxford and Wytham Woods Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium. Falk S, et al. Wellcome Open Res. 2023 Jun 23;8:275. doi: 10.12688/wellcomeopenres.19575.1. eCollection 2023. Wellcome Open Res. 2023. PMID: 38919871 Free PMC article.
See all similar articles

References

    1. Abdennur N, Mirny LA: Cooler: scalable storage for Hi-C data and other genomically labeled arrays. Bioinformatics. 2020;36(1):311–316. 10.1093/bioinformatics/btz540 - DOI - PMC - PubMed
    1. Aken BL, Ayling S, Barrell D, et al. : The ensembl gene annotation system. Database (Oxford). 2016;2016: baw093. 10.1093/database/baw093 - DOI - PMC - PubMed
    1. Allio R, Schomaker-Bastos A, Romiguier J, et al. : MitoFinder: efficient automated large-scale extraction of mitogenomic data in target enrichment phylogenomics. Mol Ecol Resour. 2020;20(4):892–905. 10.1111/1755-0998.13160 - DOI - PMC - PubMed
    1. Anderson GS: Minimum and maximum development rates of some forensically important calliphoridae (Diptera). J Forensic Sci. 2000;45(4):824–32. - PubMed
    1. Bateman A, Martin MJ, Orchard S, et al. : UniProt: the universal protein knowledgebase in 2023. Nucleic Acids Res. 2023;51(D1):D523–D531. 10.1093/nar/gkac1052 - DOI - PMC - PubMed

LinkOut - more resources