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. 2024 Nov-Dec;38(6):2735-2739.
doi: 10.21873/invivo.13752.

The Diagnostic Potential of Extracellular Vesicles Derived From the Blood Plasma of Glioblastoma Patients

Katja Döring  1 , Vesna Malinova  2 , Christoph Bettag  2 , Veit Rohde  2 , Matthias Schulz  3 , Kerstin Menck  4 , Annalen Bleckmann  4 , Claudia Binder  3 , Judith Büntzel  5
Affiliations

The Diagnostic Potential of Extracellular Vesicles Derived From the Blood Plasma of Glioblastoma Patients

Katja Döring et al. In Vivo. 2024 Nov-Dec.

Abstract

Background/aim: Biomarkers for patients suffering from glioblastoma (GBM) are scarce. Extracellular vesicles (EV) are a promising candidate for a potential biomarker. Therefore, EV concentration could be a potential biomarker of tumor burden, volume, and prognosis.

Patients and methods: Large EV (lEV) and small EV (sEV) were isolated from 36 GBM patients' blood plasma by differential centrifugation. Nanoparticle tracking was used to measure EV concentration. Quantitative analysis of tumor volume was performed by evaluating T2/FLAIR relaxation times.

Results: The mean size of lEV was 173.3 nm ± 18.2 nm, while sEV measured 148.3 ± 9.0 nm. Patients with higher lEV concentrations showed a trend towards longer overall survival (36.1 vs. 16.5 months, p=0.08). Regarding inflammatory markers, higher leukocyte count was positively correlated with higher sEV concentration (r2=0.3887, DF 21, p=0.0015). No significant relationship was found between lEV or sEV concentration and tumor volume.

Conclusion: Overall EV concentration in the peripheral blood is not a predictor of tumor volume. sEV concentration is associated with a potential pro-inflammatory metabolism.

Keywords: Extracellular vesicles; biomarker; glioblastoma; liquid biopsy.

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Conflict of interest statement

The Authors declare no conflicts of interest in relation to this study.

Figures

Figure 1
Figure 1
Patients with higher concentrations of large (A) or small extracellular vesicles (B) tend to have a longer overall survival. Kaplan–Meier curve; (A) p=0.08; HR=2.45, 95%CI=0.96-6.27. (B) p=0.11; HR=2.19, 95%CI=0.67-7.21.
See this image and copyright information in PMC

References

    1. Johnson DR, O’Neill BP. Glioblastoma survival in the United States before and during the temozolomide era. J Neurooncol. 2012;107(2):359–364. doi: 10.1007/s11060-011-0749-4. - DOI - PubMed
    1. Louis DN, Perry A, Wesseling P, Brat DJ, Cree IA, Figarella-Branger D, Hawkins C, Ng HK, Pfister SM, Reifenberger G, Soffietti R, von Deimling A, Ellison DW. The 2021 WHO Classification of Tumors of the Central Nervous System: a summary. Neuro Oncol. 2021;23(8):1231–1251. doi: 10.1093/neuonc/noab106. - DOI - PMC - PubMed
    1. Reuss DE, Mamatjan Y, Schrimpf D, Capper D, Hovestadt V, Kratz A, Sahm F, Koelsche C, Korshunov A, Olar A, Hartmann C, Reijneveld JC, Wesseling P, Unterberg A, Platten M, Wick W, Herold-Mende C, Aldape K, von Deimling A. IDH mutant diffuse and anaplastic astrocytomas have similar age at presentation and little difference in survival: a grading problem for WHO. Acta Neuropathol. 2015;129(6):867–873. doi: 10.1007/s00401-015-1438-8. - DOI - PMC - PubMed
    1. Stupp R, van den Bent MJ, Hegi ME. Optimal role of temozolomide in the treatment of malignant gliomas. Curr Neurol Neurosci Rep. 2005;5(3):198–206. doi: 10.1007/s11910-005-0047-7. - DOI - PubMed
    1. Mahmoudi K, Ezrin A, Hadjipanayis C. Small extracellular vesicles as tumor biomarkers for glioblastoma. Mol Aspects Med. 2015;45:97–102. doi: 10.1016/j.mam.2015.06.008. - DOI - PubMed

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