Preimplantation trophectoderm: A 'quick-fix' protector for embryo survival?

Abstract

The trophectoderm (TE) epithelium forms the outer layer of the mammalian blastocyst and generates the blastocoel through vectorial transport. Its differentiation during cleavage, studied mainly in mouse, is integrated with blastocyst morphogenesis with key roles for cell polarisation, asymmetric cell divisions, cell signalling, regulatory transcription factors and cellular inheritance. The TE provides a physical and cellular protection to the emerging lineages of the embryo essential for the integrity of blastocyst development. Here, two examples of TE differentiation are considered in some detail where this immediate protective function for embryo survival is assessed: (i) cellular processes from TE at the polar-mural junctional zone in the early blastocyst that later form filopodia traversing the blastocoel, and (ii) the endocytic system which matures and polarises during differentiation. Understanding the broad role for TE in regulating early morphogenesis and environmental protection of the embryo, including these two examples, have clinical as well as biological relevance.

Keywords: Blastocyst; Cytoplasmic processes; Differentiation; Endocytosis; Epithelium; Filopodia; Trophectoderm; mTORC1.