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. 2024 Oct 24:2024:5245451.
doi: 10.1155/2024/5245451. eCollection 2024.

Ganoderma tuberculosum Liquid Culture With Vineyard Pruning Extracts for Bioactive Composite Production With Antiproliferative Activity

Lucia T Angulo-Sanchez  1 , María C Cruz-Félix  1 , Max Vidal-Gutiérrez  2 , Heriberto Torres-Moreno  3 , Óscar A Muñoz-Bernal  4 , Emilio Álvarez-Parrilla  4 , Ramón E Robles-Zepeda  5 , Osiris Álvarez-Bajo  6 , Aldo Gutiérrez  1 , Martín Esqueda  1
Affiliations

Ganoderma tuberculosum Liquid Culture With Vineyard Pruning Extracts for Bioactive Composite Production With Antiproliferative Activity

Lucia T Angulo-Sanchez et al. Adv Pharmacol Pharm Sci. .

Abstract

Ganoderma species have been studied for their pharmacological approaches, such as anticancer, antitumor, antiproliferative, and antioxidant activity. Elicitors are used to increase Ganoderma bioactive composite production. This study aims to evaluate the antiproliferative activity of ethanolic extracts from mycelium of Ganoderma tuberculosum (G. tuberculosum) grown in a liquid medium with vineyard pruning waste (VPW) extracts as elicitors. Ethanolic and aqueous VPW extracts contain resveratrol dimer 4, resveratrol tetramer 1, and naringenin, while toluene and chloroform extracts contain tetradecanoic acid, hexadecanoic acid, and octadecanoic acid. Polar and nonpolar extracts could be promising elicitors for increasing bioactive molecules. Catechin gallate showed the highest correlation (r = 0.66) with biomass. Mycelial ethanolic extracts of G. tuberculosum (native strain from the Sonoran Desert) and Ganoderma lucidum (G. lucidum) (control) were analyzed by ESI-IT-MS, and 27 molecules were identified for the two species. They showed antiproliferative activity against the A549 and C-33 A cell lines but not for ARPE-19. G. tuberculosum culture with VPW had quinic acid, ganodermenonol, ganoderic acid I (GA-I), C2 (GA-C2), and 20-hydroxylucidenic acid P, among others. Molecular docking of ganodermenonol, GA-I, and GA-C2 demonstrates significant interaction with tumor necrotic factor (TNF-α). These ethanolic extracts of Ganoderma are promising sources of bioactive triterpenoids. Their antiproliferative activity did not change between species or treatment. Likewise, the G. tuberculosum and G. lucidum extracts only affected cancer cell lines. This property seems promising for pharmacological applications of these fungal extracts.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Correlation of vineyard pruning wastes extracts composites vs. biomass. G = group.
Figure 2
Figure 2
Morphological changes induced by Ganoderma tuberculosum extract of control at 15 d (GTC15) on A549 and ARPE-19 cell lines. (A) A549 cell line. (B) A549 + GTC15 24 h–200 µg/mL-20x. (C) ARPE-19 cell line. (D) ARPE-19 + GTC15 24 h–200 µg/mL-20x. Black arrows: formation of vacuoles; white arrows: cell debris.
Figure 3
Figure 3
Composites identified in the extract of Ganoderma lucidum and G. tuberculosum.
Figure 4
Figure 4
Chromatogram of Ganoderma tuberculosum and G. lucidum ethanolic extracts by ESI-MS in positive and negative mode, and APCI negative mode. (a) Chromatogram of ethanolic extracts of G. tuberculosum in control and treatment with VPW, (b) chromatogram of ethanolic extracts of G. lucidum in control and treatment with VPW.
Figure 5
Figure 5
Molecular docking of ganodermenonol (a), ganoderic acid I (b), and ganoderic acid C2 (c) with TNF-α protein. Aqua: shows the H bond.
See this image and copyright information in PMC

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