Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Nov;59(11):e14740.
doi: 10.1111/rda.14740.

Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm

Reihaneh Nateghi  1 , Reza Masoudi  2 , Nader Asadzadeh  2
Affiliations

Supplementing the Beltsville Extender With Mitoquinol Improves the Quality and Fertility Potential of the Rooster's Cooled Sperm

Reihaneh Nateghi et al. Reprod Domest Anim. 2024 Nov.

Abstract

Supplementing freeze diluents with certain antioxidants can maintain the quality of chilled sperm. The present study was an attempt to investigate the effect of Beltsville extender supplementation with the mitochondrial-targeted antioxidant 'Mitoquinol' on the quality parameters and fertility potential of rooster sperm during the cooling process. Semen samples were diluted in Beltsville extender, divided into five groups, and supplemented with 0, 1, 10, 100 and 1000 nM Mitoquinol. Samples were stored at 5°C for up to 50 h and then assayed for sperm motility, viability, mitochondrial function, membrane integrity and malondialdehyde concentration after 0, 25 and 50 h of cooling. To assess reproductive performance, artificial insemination was performed using sperm cooled for 25 h. The results showed no differences between groups at the beginning time. Extender supplementation with 10 and 100 nM Mitoquinol resulted in an improvement in total motility, progressive motility, membrane integrity, mitochondrial function and viability (p ≤ 0.05), as well as a lower malondialdehyde concentration (p ≤ 0.05) in comparison to the other groups during 25 and 50 h storage. Fertility rates were higher when roosters were inseminated with semen samples supplemented with 10 and 100 nM Mitoquinol, compared to the control group. Therefore, supplementing Beltsville extender with Mitoquinol (10 and 100 nM) effective in improving the quality and fertility potential of cooled rooster sperm.

Keywords: Mitoquinol; cooling; fertility; rooster; sperm.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Asadzadeh, N., Z. Abdollahi, S. Esmaeilkhanian, and R. Masoudi. 2021. “Fertility and Flow Cytometry Evaluations of Ram Frozen Semen in Plant‐Based Extender Supplemented With Mito‐TEMPO.” Animal Reproduction Science 233: 106836. https://doi.org/10.1016/j.anireprosci.2021.106836.
    1. Askarianzadeh, Z., M. Sharafi, and M. A. Karimi Torshizi. 2018. “Sperm Quality Characteristics and Fertilization Capacity After Cryopreservation of Rooster Semen in Extender Exposed to a Magnetic Field.” Animal Reproduction Science 198: 37–46. https://doi.org/10.1016/j.anireprosci.2018.08.043.
    1. Burrows, W. H., and J. P. J. P. S. Quinn. 1937. “The Collection of Spermatozoa From the Domestic Fowl and Turkey.” Collection of Spermatozoa From the Domestic Fowl and Turkey 16: 19–24.
    1. Cerolini, S., L. Zaniboni, A. Maldjian, and T. Gliozzi. 2006. “Effect of Docosahexaenoic Acid and Alpha‐Tocopherol Enrichment in Chicken Sperm on Semen Quality, Sperm Lipid Composition and Susceptibility to Peroxidation.” Theriogenology 66, no. 4: 877–886. https://doi.org/10.1016/j.theriogenology.2006.02.022.
    1. Esterbauer, H., and K. H. Cheeseman. 1990. “Determination of Aldehydic Lipid Peroxidation Products: Malonaldehyde and 4‐Hydroxynonenal.” Methods in Enzymology 186: 407–421. https://doi.org/10.1016/0076‐6879(90)86134‐h.

LinkOut - more resources