High prevalence of multidrug-resistant Escherichia coli in retail aquatic products in China and the first report of mcr-1-positive extended-spectrum β-lactamase-producing E. coli ST2705 and ST10 in fish

Abstract

Contamination of food by multidrug-resistant (MDR) bacteria is a potential threat to consumers. Aquatic products are increasingly consumed due to their high value and rich nutrient. Nevertheless, the prevalence of multi-drug resistant (MDR) E. coli in retail aquatic products has not been systematically investigated in China. In this study, we conducted a national investigation on the prevalence of E. coli and MDR E. coli in retail aquatic products and the characteristics of the MDR E. coli isolates. A total of 849 samples consisting of 680 fish, 143 shrimp, and 26 shellfish were purchased from markets in 39 cities in China and investigated for the presence of E. coli. Overall, 340 (40.0 %) and 169 (19.9 %) samples were contaminated with E. coli and MDR E. coli, indicating poor hygiene conditions of retail aquatic products. A total of 190 MDR E. coli were isolated, which were recovered from 38.5 % shellfish, 20.1 % shrimp, and 19.0 % fish. Thirty-two ESBL-producing E. coli were identified from 3.5 % fish, 3.5 % shrimp, and 3.8 % shellfish. The MDR E. coli isolates showed a high prevalence of resistance to tetracycline (93.7 %), trimethoprim-sulfamethoxazole (78.9 %), ampicillin (78.4 %), chloramphenicol (72.1 %), nalidixic acid (73.2 %), cephalothin (65.3 %), and streptomycin (65.8 %). Resistances to kanamycin (42.1 %), gentamicin (37.9 %), ciprofloxacin (42.6 %), and norfloxacin (45.8 %) were also common. Further, 15.3 % and 8.4 % of the isolates were resistant to cefotaxime and ceftazidime, respectively. Four isolates were resistant to colistin. More than 85.0 % of the isolates were resistant to 5-15 antibiotics. Of the antibiotic resistance genes, TEM-1, tetA, strA/B, sul2, aadA, floR, and qnrS were the most prevalent, followed by sul1, aac(6')-Ib, oqxA/B, cmlA and aphA1. Six CTX-M-types were found, among which CTX-M-55 and CTX-M-14 were predominant. All 4 colistin-resistant isolates carried the mcr-1 gene, of which three were ESBL strains. WGS indicated that the mcr-1 gene was located on two types of plasmids (IncHI2 and IncX4). Conjugation experiments showed the mcr-1 gene could be transferred to E. coli C600. To our knowledge, this is the first report of mcr-1-positive ESBL E. coli (ST10 and ST2705) in retail aquatic products. The emergence of such strains poses a serious threat to consumers, with the potential to spread resistance genes to humans. Our results indicate that retail aquatic products are important reservoirs of MDR E. coli and facilitate the dissemination of the resistance genes. Continuous surveillance and interventions of restricting antibiotic use in aquatic environments should also be applied to reduce the prevalence of MDR bacteria.

Keywords: Antimicrobial resistance; Aquatic products; Colistin; Escherichia coli; Extended-spectrum β-lactamase–producing; Whole-genome sequencing.