Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Nov 4;14(1):26679.
doi: 10.1038/s41598-024-77130-1.

Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains

Hanan Balto  1 , Reem Barakat  2 , Sumaya Basudan  1 , Ghazal Fakeeha  1 , Sarah R Alharbi  3 , Rahaf Almohareb  4
Affiliations

Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains

Hanan Balto et al. Sci Rep. .

Abstract

This study evaluated the antibiofilm efficacy of a calcium silicate-based intracanal medicament (Bio-C Temp) against Fusobacterium nucleatum (F. nucleatum). Dentin slices (n = 88) were inoculated with F. nucleatum biofilms and divided into experimental groups (n = 24 each) treated with Bio-C Temp, triple antibiotic paste (TAP), or Ca(OH)2 for 1 or 2 weeks. Untreated dentin slices exposed to saline served as a positive control, while sterile dentin slices incubated anaerobically served as a negative control. Bacterial viability was assessed using confocal laser scanning microscopy after staining with LIVE/DEAD® BacLight™ dye. Samples treated with TAP demonstrated the highest percentage of dead bacteria (95%). The antibiofilm effect of TAP and Bio-C Temp significantly increased from Week 1 to Week 2. At Week 2, samples treated with Bio-C Temp showed a significantly higher percentage of dead bacteria (75.25%) than those treated with Ca(OH)2 (58.65%). TAP showed superior efficacy against F. nucleatum biofilms. After an application time of 2 weeks, the antibiofilm efficacy of Bio-C Temp was superior to that of Ca(OH)2.

Keywords: Bio-C temp; Calcium hydroxide; Intracanal medicament; Triple antibiotic paste.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Representative images of specimens from each group at different treatment stages. (A) SEM image of pretreatment specimen following 3 weeks of incubation. (B, C, D, E) CLSM images of specimens from each group treated with assigned material, green fluorescence indicates live bacteria while red indicates dead bacteria. (B) After 1 week application of saline as control. (C1) 1-week application of TAP, (C2) 2-week application of TAP. (D1) 1-week application of Ca(OH)2, (D2) 2-week application of Ca(OH)2. (E1) 1-week application of Bio-C Temp, (E2) 2-week application of Bio-C Temp.
Fig. 2
Fig. 2
Comparison of median percentage of dead bacteria for each experimental group after one and two weeks of application of assigned material.
See this image and copyright information in PMC

References

    1. Nair, P. N. R. On the causes of persistent apical periodontitis: A review. Int. Endod. J.39, 249–281 (2006). - DOI - PubMed
    1. Siqueira, J. F. J. & Rôças, I. N. Clinical implications and microbiology of bacterial persistence after treatment procedures. J. Endod.34, 1291-1301.e3 (2008). - DOI - PubMed
    1. Bystrom, A., Claesson, R. & Sundqvist, G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod. Dent. Traumatol.1, 170–175 (1985). - DOI - PubMed
    1. Kim, D. & Kim, E. Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: A literature review—Part I. In vitro studies. Restor. Dent. Endod.39, 241–252 (2014). - DOI - PMC - PubMed
    1. Mohammadi, Z. & Dummer, P. M. H. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int. Endod. J.44, 697–730 (2011). - DOI - PubMed

MeSH terms