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. 2024 Nov 4;14(1):26679.
doi: 10.1038/s41598-024-77130-1.

Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains

Hanan Balto  1 , Reem Barakat  2 , Sumaya Basudan  1 , Ghazal Fakeeha  1 , Sarah R Alharbi  3 , Rahaf Almohareb  4
Affiliations

Antibiofilm efficacy of a calcium silicate-based intracanal medicament against Fusobacterium nucleatum strains

Hanan Balto et al. Sci Rep. .

Abstract

This study evaluated the antibiofilm efficacy of a calcium silicate-based intracanal medicament (Bio-C Temp) against Fusobacterium nucleatum (F. nucleatum). Dentin slices (n = 88) were inoculated with F. nucleatum biofilms and divided into experimental groups (n = 24 each) treated with Bio-C Temp, triple antibiotic paste (TAP), or Ca(OH)2 for 1 or 2 weeks. Untreated dentin slices exposed to saline served as a positive control, while sterile dentin slices incubated anaerobically served as a negative control. Bacterial viability was assessed using confocal laser scanning microscopy after staining with LIVE/DEAD® BacLight™ dye. Samples treated with TAP demonstrated the highest percentage of dead bacteria (95%). The antibiofilm effect of TAP and Bio-C Temp significantly increased from Week 1 to Week 2. At Week 2, samples treated with Bio-C Temp showed a significantly higher percentage of dead bacteria (75.25%) than those treated with Ca(OH)2 (58.65%). TAP showed superior efficacy against F. nucleatum biofilms. After an application time of 2 weeks, the antibiofilm efficacy of Bio-C Temp was superior to that of Ca(OH)2.

Keywords: Bio-C temp; Calcium hydroxide; Intracanal medicament; Triple antibiotic paste.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Representative images of specimens from each group at different treatment stages. (A) SEM image of pretreatment specimen following 3 weeks of incubation. (B, C, D, E) CLSM images of specimens from each group treated with assigned material, green fluorescence indicates live bacteria while red indicates dead bacteria. (B) After 1 week application of saline as control. (C1) 1-week application of TAP, (C2) 2-week application of TAP. (D1) 1-week application of Ca(OH)2, (D2) 2-week application of Ca(OH)2. (E1) 1-week application of Bio-C Temp, (E2) 2-week application of Bio-C Temp.
Fig. 2
Fig. 2
Comparison of median percentage of dead bacteria for each experimental group after one and two weeks of application of assigned material.
See this image and copyright information in PMC

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