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. 2025:2869:157-168.
doi: 10.1007/978-1-0716-4204-7_16.

Chromatin Immunoprecipitation from Shoot Apices and Young Panicles of Rice

Katsutoshi Tsuda  1   2
Affiliations

Chromatin Immunoprecipitation from Shoot Apices and Young Panicles of Rice

Katsutoshi Tsuda. Methods Mol Biol. 2025.

Abstract

Chromatin immunoprecipitation (ChIP) assay is a standard method to examine protein-DNA interactions in vivo. Here, I describe a basic procedure for ChIP assay in rice shoot apices using an antibody against the homeodomain transcription factor Oryza sativa homeobox1 (OSH1). I also introduce the tips to overcome several obstacles when performing this assay for the first time and potential pitfalls that could impact the result.

Keywords: Antibody; Chromatin immunoprecipitation (ChIP); Immunoblotting; Shoot meristem.

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References

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