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. 1979 Jul;92(3):741-7.
doi: 10.1093/genetics/92.3.741.

Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5

K J Shaw, C M Berg

Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5

K J Shaw et al. Genetics. 1979 Jul.

Abstract

The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.

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