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. 2024 Sep 20;55(5):1254-1263.
doi: 10.12182/20240960302.

[Expression of circRNA_051778 in Lung Adenocarcinoma-Associated Malignant and Tuberculous Pleural Effusions and Its Clinical Significance]

[Article in Chinese]
Zhishan Ye  1 , Xueping Nong  1 , Yanyun Wang  1 , Guanglu Che  1 , Bin Zhou  1 , Jianhua Huang  1 , Lin Zhang  1   2
Affiliations

[Expression of circRNA_051778 in Lung Adenocarcinoma-Associated Malignant and Tuberculous Pleural Effusions and Its Clinical Significance]

[Article in Chinese]
Zhishan Ye et al. Sichuan Da Xue Xue Bao Yi Xue Ban. .

Abstract
in English, Chinese

Objective: To investigate the expression and clinical significance of circular RNA (circRNA) 051778 in lung adenocarcinoma-malignant pleural effusion (LA-MPE) and tuberculous pleural effusion (TPE).

Methods: This is a cross-sectional study. A total of 212 patients were recruited from the Jiangxi Chest Hospital between October 2018 and September 2019, and their pleural effusion samples and/or plasma samples were collected. The exosomal circRNA profile was sketched by circRNA microarray. Differentially expressed circRNAs (DECs) were verified by droplet digital PCR. In addition, a putative circRNA-miRNA-mRNA network was constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to predict the functions of the DECs. The diagnostic value of circRNA_051778 was evaluated by binary logistic regression and receiver operating characteristic curve.

Results: The expression level of circRNA_051778 in the LA-MPE samples was (3.92±0.48) copies/100 ng cDNA, while that in the TPE samples was (21.53±2.22) copies/100 ng cDNA. Compared to that in the TPE samples, circRNA_051778 was significantly downregulated in the LA-MPE samples (P<0.001). The potential targets of circRNA_051778 were enriched in positive regulation of GTPase activity, cytoplasm, protein binding, and cancer-related pathways. The area under the curve (AUC) for the combined assessment of circRNA_051778 with liquid-based thin-layer cytology (TCT), erythrocyte sedimentation rate (ESR), and tuberculosis antibody (TBA) was 0.98 (95% confidence interval: 0.97-1.00), with the sensitivity being 88.0% and the specificity being 100.0%.

Conclusion: Exosomal circRNA_051778 is downregulated in LA-MPE. According to the findings from the GO and KEGG analyses, exosomal circRNA_051778 may play a role in cancer development and has the potential to serve as a marker for differential diagnostic of LA-MPE and TPE when it is used in combination with TCT, ESR, and TBA.

目的: 分析circRNA_051778在肺腺癌性恶性胸腔积液（LA-MPE）和结核性胸腔积液（TPE）样本中的临床意义。

方法: 本研究为横断面研究。2018年10月–2019年9月间于江西省胸科医院共募集212例患者，收集患者入院第1天胸腔积液和/或血浆。使用circRNA微阵列分析LA-MPE和TPE样本中的外泌体环状RNA（circRNAs），通过微滴式数字PCR验证差异表达环状RNA（DECs）。此外，构建可能的circRNA-miRNA-mRNA网络，并进行了GO（Gene Ontology）分析和KEGG（Kyoto Encyclopedia of Genes and Genomes）通路分析，以预测DECs的功能。通过二分类逻辑回归和受试者工作特征曲线评估circRNA_051778的诊断价值。

结果: circRNA_051778的表达水平在LA-MPE样本中为（3.92±0.48）拷贝数/100 ng cDNA，在TPE样本中为 （21.53±2.22 ）拷贝数/100 ng cDNA。与TPE相比，LA-MPE样本中的circRNA_051778下调（P<0.001）。circRNA_051778的潜在靶标富集于GTPase活性正调控、细胞质、蛋白结合和癌症相关通路中。circRNA_051778与液基薄层细胞学检查（TCT）、红细胞沉降率（ESR）和结核抗体（TBA）联合检测的曲线下面积为0.98（95%置信区间：0.97~ 1.00），敏感性为88.0%，特异性为100.0%。

结论: 外泌体中的circRNA_051778在LA-MPE中下调，GO和KEGG分析结果显示其可能在癌症的发展中发挥作用，与TCT、ESR、TBA联合有望作为LA-MPE和TPE鉴别诊断标志物。

Keywords: Circular RNA; Exosome; Lung adenocarcinoma; Pleural effusion; Tuberculosis.

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Conflict of interest statement

利益冲突 本文作者张林是本刊主编。该文在编辑评审过程中所有流程严格按照期刊政策进行，且未经其本人经手处理。除此之外，所有作者声明不存在利益冲突。

Figures

图 1
图 1
The expression of circRNA_051778 in 100 LA-MPE samples and 112 TPE samples 100个肺腺癌性恶性胸腔积液样本和112个结核性胸腔积液样本中circRNA_051775表达情况
图 2
图 2
The expression of circRNA_051778 in matched plasma and PE samples from the lung adenocarcinoma and tuberculosis groups circRNA_051778在肺腺癌和肺结核配对的血浆和PE样本中的表达
图 3
图 3
The top 15 significantly enriched KEGG pathways for the 2852 predicted mRNA targets of circRNA_051778 circRNA_051778的2852个预测mRNA靶点的前15个显著富集的KEGG通路
图 4
图 4
The ROC curves of circRNA_051778, TCT, the combination of TCT, ESR and TBA, and the combination of TCT, ESR, TBA, and circRNA_051778 circRNA_051778，TCT，TCT、ESR和TBA联合，TCT、ESR、TBA和circRNA_051778联合的ROC曲线
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