Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 May;103(3):281-288.
doi: 10.1111/aos.16791. Epub 2024 Nov 7.

Graft detachment rates in surgeon-cut and pre-cut tissue for DSAEK transported in different mediums

Thorbjörg Olafsdottir  1 , Faisal Hootak  2 , Carina Forslund  3 , Eydis Olafsdottir  1 , Ingemar Gustafsson  1
Affiliations

Graft detachment rates in surgeon-cut and pre-cut tissue for DSAEK transported in different mediums

Thorbjörg Olafsdottir et al. Acta Ophthalmol. 2025 May.

Abstract

Purpose: To analyse graft detachment rates after Descemet stripping automated endothelial keratoplasty (DSAEK) using surgeon-cut grafts and pre-cut grafts transported in different mediums.

Methods: A retrospective study of graft detachment rates including 265 surgeries (240 patients) performed between 2019 and 2023. The DSAEK grafts were either surgeon-cut (n = 135) or pre-cut and transported in a dextran-containing medium (n = 82) or in a dextran-free medium (n = 48). All surgeries were performed by a single surgeon (I.G.). The surgeries were categorised according to whether the recipients' eyes had, or did not have, factors associated with an increased risk of graft detachment. Detachment rates were compared for all patients receiving the different kinds of graft, and only for those without risk factors.

Results: A significantly higher detachment rate was found among patients receiving pre-cut grafts transported in a dextran-containing medium (32.9%), compared to surgeon-cut grafts (14.1%, p = 0.002), and pre-cut grafts transported in a dextran-free medium (6.3%, p = 0.002) for all recipients (those with and without risk factors for graft detachment). When analysing recipients without risk factors for graft detachment, the detachment rate was still significantly higher with pre-cut tissue transported in a dextran-containing medium (26.9%), compared to surgeon-cut tissue (8.3%, p = 0.002) and pre-cut tissue transported in a dextran-free medium (3.6%, p = 0.01).

Conclusions: The use of pre-cut DSAEK tissue transported in a dextran-containing medium significantly increases the risk of graft detachment requiring re-bubbling.

Keywords: DSAEK; bullous keratopathy; fuchs dystrophy; lamellar keratoplasty.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
Detachment rates (%) in the three groups including all patients, with and without risk factors.
FIGURE 2
FIGURE 2
Detachment rates (%) in the three groups when considering only patients without risk factors.
See this image and copyright information in PMC

References

    1. Bhogal, M.S. , Angunawela, R.I. , Bilotti, E. , Eames, I. & Allan, B.D. (2012) Theoretical, experimental, and optical coherence tomography (OCT) studies of graft apposition and adhesion in Descemets stripping automated endothelial keratoplasty (DSAEK). Investigative Ophthalmology & Visual Science, 53, 3839–3846. - PubMed
    1. Cardascia, N. , Pastore, V. , Bini, V. , Lategola, M.G. & Alessio, G. (2020) Graft detachment after Descemet's stripping automated endothelial keratoplasty in bullous keratopathy and Fuchs dystrophy. Medical Hypothesis, Discovery and Innovation in Ophthalmology, 9, 15–22. - PMC - PubMed
    1. Chaurasia, S. , Vaddavalli, P.K. , Ramappa, M. , Garg, P. & Sangwan, V.S. (2011) Clinical profile of graft detachment and outcomes of rebubbling after Descemet stripping endothelial keratoplasty. The British Journal of Ophthalmology, 95, 1509–1512. - PubMed
    1. Chen, E.S. & Naseri, A. (2009) Presoaking of donor corneas for the reduction of postoperative dislocation after descemet stripping endothelial keratoplasty. American Journal of Ophthalmology, 148, 471 author reply 471‐472. - PubMed
    1. Dapena, I. , Ham, L. , Droutsas, K. , van Dijk, K. , Moutsouris, K. & Melles, G.R. (2011) Learning curve in Descemet's membrane endothelial keratoplasty: first series of 135 consecutive cases. Ophthalmology, 118, 2147–2154. - PubMed

LinkOut - more resources