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. 2024 Oct 24:11:1474870.
doi: 10.3389/fmed.2024.1474870. eCollection 2024.

Animal models of chronic obstructive pulmonary disease: a systematic review

Tiantian Feng #  1 , Juan Cao #  2 , Xiaoting Ma  3 , Xinhua Wang  1 , Xiaolong Guo  1 , Na Yan  1 , Chunling Fan  4 , Shisan Bao  1 , Jingchun Fan  1
Affiliations

Animal models of chronic obstructive pulmonary disease: a systematic review

Tiantian Feng et al. Front Med (Lausanne). .

Abstract

Objective: Experimental animal models have been used for decades to study the development and progression of chronic obstructive pulmonary disease (COPD). However, there is a lack of methods for constructing animal models of COPD for optimal modelling. This systematic literature review (SLR) aimed to assess the various methods used to establish COPD animal models, highlight their advantages and limitations, and explore more optimized approaches for establishing such models.

Methods: A systematic search was performed in four English databases (PubMed, Embase, Web of Science, and the Cochrane Library) and four Chinese databases (Chinese Biomedical Literature Database, China National Knowledge Infrastructure, China Science and Technology Journal Database, and Wanfang Database). Of the 8,015 retrieved full-text manuscripts, 453 were selected.

Results: Smoking (n = 140), smoking combined with lipopolysaccharide (LPS) (n = 275), smoking combined with protease drip (PPE) (n = 10), smoking combined with bacteria (n = 23), and smoking combined with particulate matter (PM2.5) (n = 5) were the most used methods for establishing animal models of COPD. Rats and mice were the most frequently selected experimental animals, with male animals accounting for 79.47% of the total. A total of 92.49 and 29.14% of the articles reviewed considered lung pathology of experimental animals only and lung pathology and lung function tests, respectively.

Conclusion: Our review suggests that the best way to establish an animal model of COPD is to combine smoking with LPS. Although findings from animal models of COPD cannot be directly extrapolated to human COPD, they could provide useful tools for further investigation into human COPD disease.

Systematic review registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023407555, Identifier PROSPERO CRD42023407555.

Keywords: COPD; animal models; modelling method; review–systematic; smoke + LPS.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Figures

Figure 1
Figure 1
The flow diagram for study selection.
Figure 2
Figure 2
The number of the included studies sorted by the year of publication.
See this image and copyright information in PMC

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