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. 2024 Nov-Nov;16(21-22):1337-1350.
doi: 10.1080/17501911.2024.2419362. Epub 2024 Nov 8.

DNA methylation analysis of multiple genes in thymic epithelial tumors

Vanessa Nicolì  1 , Marianna Giangreco  1 , Eleonora Pardini  1 , Iacopo Petrini  1 , Diana Bacchin  2 , Vittorio Aprile  2 , Franca Melfi  3 , Marco Lucchi  2 , Melania Guida  4 , Roberta Ricciardi  4 , Michelangelo Maestri  4 , Martina Lari  1 , Lucia Migliore  1   5 , Andrea Stoccoro  1   5 , Fabio Coppedè  1   5
Affiliations

DNA methylation analysis of multiple genes in thymic epithelial tumors

Vanessa Nicolì et al. Epigenomics. 2024 Nov-Nov.

Abstract

Aim: To investigate DNA methylation levels of a panel of genes in thymic epithelial tumors (TETs).Materials & methods: We selected 15 genes among the most promising epigenetic biomarkers of TETs and evaluated their methylation levels in 71 TET samples.Results: thymic carcinomas (TCs) showed hypermethylation of GHSR and ELF3 genes and reduced IL1RN methylation levels compared with thymomas (TMs) and healthy thymic tissues. RAG1 was hypomethylated in TMs compared with healthy thymic tissues. No difference in the methylation levels of the investigated genes was seen among TM stages and subtypes. No changes in blood methylation levels of the investigated genes were seen among TET subtypes.Conclusion: The present study confirms GHSR, ELF3, IL1RN and RAG1 as TET epigenetic biomarkers.

Keywords: DNA methylation; GHSR; IL1RN; RAG1; thymic carcinoma; thymic epithelial tumors; thymoma.

Plain language summary

[Box: see text].

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Conflict of interest statement

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

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