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. 2024 Oct 27;25(21):11528.
doi: 10.3390/ijms252111528.

Genetic Associations of Plasminogen Activator Inhibitor-1-Related miRNA Variants with Coronary Artery Disease

Yong Hyun Ha  1 , Jung Hoon Sung  2 , Chang Soo Ryu  1 , Eun Ju Ko  1 , Hyeon Woo Park  1 , Han Sung Park  1 , Ok Joon Kim  3 , In Jai Kim  2 , Nam Keun Kim  1
Affiliations

Genetic Associations of Plasminogen Activator Inhibitor-1-Related miRNA Variants with Coronary Artery Disease

Yong Hyun Ha et al. Int J Mol Sci. .

Abstract

Coronary artery disease (CAD) is one of the most common types of cardiovascular disease and can lead to a heart attack as plaque gradually builds up inside the coronary arteries, blocking blood flow. Previous studies have shown that polymorphisms in the PAI-1 gene are associated with CAD; however, studies of the PAI-1 3'-untranslated region, containing a miRNA binding site, and the miRNAs that interact with it, are insufficient. To investigate the association between miRNA polymorphisms and CAD in the Korean population based on post-transcriptional regulation, we genotyped five polymorphisms in four miRNAs targeting the 3'-untranslated region of PAI-1 using real-time PCR and TaqMan assays. We found that the mutant genotype of miR-30c rs928508 A > G was strongly associated with increased CAD susceptibility. In a genotype combination analysis, the combination of the homozygous mutant genotype (GG) of miR-30c rs928508 with the wild-type genotype (GG) of miR-143 rs41291957 resulted in increased risk for CAD. Also, in an allele combination analysis, the combination of the mutant allele (G) of miR-30c rs928508 and the wild-type allele (G) of miR-143 rs41291957 resulted in increased risk for CAD. Furthermore, metabolic syndrome and diabetes mellitus showed synergistic effects on CAD risk when combined with miR-30c rs928508. These results can be applied to identify CAD prognostic biomarkers among miRNA polymorphisms and various clinical factors.

Keywords: coronary artery disease; microRNA; plasminogen activator inhibitor-1.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Analysis of synergistic effects on coronary artery disease risk between miR-30c rs928508 and (A) metabolic syndrome (MetS), (B) diabetes mellitus (DM), (C) fasting blood sugar (FBS), and (D) folate.
See this image and copyright information in PMC

References

    1. Park H.S., Kim I.J., Kim E.G., Ryu C.S., Lee J.Y., Ko E.J., Park H.W., Sung J.H., Kim N.K. A study of associations between CUBN, HNF1A, and LIPC gene polymorphisms and coronary artery disease. Sci. Rep. 2020;10:16294. doi: 10.1038/s41598-020-73048-6. - DOI - PMC - PubMed
    1. MacMahon S., Peto R., Cutler J., Collins R., Sorlie P., Neaton J., Abbott R., Godwin J., Dyer A., Stamler J. Blood pressure, stroke, and coronary heart disease. Part 1, Prolonged differences in blood pressure: Prospective observational studies corrected for the regression dilution bias. Lancet. 1990;335:765–774. doi: 10.1016/0140-6736(90)90878-9. - DOI - PubMed
    1. Mulcahy R. The health benefits of smoking cessation. Ir. Med. J. 1990;83:45–46. - PubMed
    1. Stamler J., Vaccaro O., Neaton J.D., Wentworth D. Diabetes, other risk factors, and 12-yr cardiovascular mortality for men screened in the Multiple Risk Factor Intervention Trial. Diabetes Care. 1993;16:434–444. doi: 10.2337/diacare.16.2.434. - DOI - PubMed
    1. Verschuren W.M., Jacobs D.R., Bloemberg B.P., Kromhout D., Menotti A., Aravanis C., Blackburn H., Buzina R., Dontas A.S., Fidanza F., et al. Serum total cholesterol and long-term coronary heart disease mortality in different cultures. Twenty-five-year follow-up of the seven countries study. JAMA. 1995;274:131–136. doi: 10.1001/jama.1995.03530020049031. - DOI - PubMed

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