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. 2024 Oct 21:24:101911.
doi: 10.1016/j.fochx.2024.101911. eCollection 2024 Dec 30.

A clenbuterol detection method based on magnetic separation up-conversion fluorescent probe

Xin-Jie Song  1 , Fei Ye  1 , Yao Zhang  1 , Juan Sun  1 , Xuping Shentu  2 , Xiaoping Yu  2 , Wei Li  3 , Yuan-Feng Wu  1
Affiliations

A clenbuterol detection method based on magnetic separation up-conversion fluorescent probe

Xin-Jie Song et al. Food Chem X. .

Abstract

In this study, a fluorescence detection method combining aptamer-modified up-conversion nanoparticles (UCNPs) and magnetic nanoparticles (MNPs) was developed for detection of Clenbuterol (CLB). The aptamer-modified magnetic NPs captured CLB, which reacted with the aptamer-modified UCNPs and generated a sandwich complex. The aptamer-modified UCNPs acted as a fluorescence source. The MNP-CLB-UCNP complex was retrieved from the solution using an magnetic field, and the fluorescence intensity was detected by fluorescence spectrophotometry with excitation and emission spectra at 980 nm and in the 400-800 nm region, respectively. The results showed that the fluorescence intensity gradually increased with increasing concentrations of CLB with a good specify. The method was highly sensitive for the quantification of CLB, with a limit of detection of 0.304 ng mL-1. The recovery rate of CLB from pork samples ranged from 84 % to 94.87 %. This fluorescence method enables the sensitive, precise, and accurate quantification of CLB residues in pork samples.

Keywords: Aptamer; Clenbuterol; Detection method; Magnetic nanoparticles; Up-conversion nanoparticles.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Schematic of illustration the principle of detection of clenbuterol.
Fig. 2
Fig. 2
(a) FTIR spectra of amino-Fe3O4; (b) XRD spectra of amino-Fe3O4; (c) SEM images of amino ferro-ferric oxide; (d) SEM images of up-conversion nanoparticles; (e) TEM images of surface modified up-conversion nanoparticles; (f) Infrared spectra of up-conversion nanoparticles and surface modified up-conversion nanoparticles.
Fig. 3
Fig. 3
The UV absorption spectra of (a)Aptamer, Apt-UCNPs and amino-UCNPs (b)Aptamer, Apt-NH2-Fe3O4 and NH2-Fe3O4.
Fig. 4
Fig. 4
(a)Fluorescence detection results of clenbuterol at different concentrations; (b) Linear relationship between clenbuterol concentration and fluorescence intensity; (c) The fluorescence intensity of each concentration of CLB was added to the actual pork sample.
Fig. 5
Fig. 5
(a) Fluorescence intensity test results of blank, estradiol, enrofloxacin, tetracycline, salbutamol, potassium sorbate and CLB at different concentrations; (b) Specificity of the detection methods.
Fig. 6
Fig. 6
The linear relationship between the area ratio of clenbuterol to metoprolol and the concentration ratio.
See this image and copyright information in PMC

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