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Published Erratum
. 2024 Nov 19;121(47):e2420361121.
doi: 10.1073/pnas.2420361121. Epub 2024 Nov 11.

Correction for Sigismund et al., Clathrin-independent endocytosis of ubiquitinated cargos

No authors listed
Published Erratum

Correction for Sigismund et al., Clathrin-independent endocytosis of ubiquitinated cargos

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 6.
Fig. 6.
Effects of multiple KDs on internalization. (A) (Top) Lysates from HeLa KD stable clones (+) for eps15/eps15R, epsin, or eps15/eps15R/epsin (triple KD) or from control clones obtained with mismatched oligos (–) were immunoblotted as indicated. In the eps15R IB, the control (−siRNA) lane of the eps15/R KD and epsin KD panels is the same, because the samples were run on the same gel, in the order epsin KD, control, eps15/R KD. (Middle) EGFR levels were comparable in all clones. (Bottom) The clathrin levels in the clathrin KDs. (B) (Upper) The indicated HeLa KD clones were transfected with a HA-EGFR/Ubmut chimera (SI Appendix, Materials and Methods) to circumvent the presence of endogenous EGFR. Internalization was assessed with an anti-HA antibody. Data are expressed as the percentage of cells in which internalization was inhibited. (Lower) A HeLa triple KD clone expressing HA-EGFR/Ubmut was transfected with the indicated eps15mut constructs (SI Appendix, Materials and Methods). Internalization assays and bar graph are as in Upper. Actual images of these experiments are in Fig. 11. (C) HeLa clones harboring the indicated KDs were incubated with 1.5 ng/mL 125I-EGF. The rate of internalization is expressed as internalized/surface-bound radioactivity. *P = 0.0164; **P = 0.003; linear regression analysis. (D) The indicated HeLa KD clones were treated with filipin (+) or mock treated (–), followed by incubation with 1.5 or 20 ng/mL 125I-EGF for 6 min at 37 °C. The rate of internalization is expressed as internalized/surface-bound radioactivity.
See this image and copyright information in PMC

Erratum for

  • Clathrin-independent endocytosis of ubiquitinated cargos.
    Sigismund S, Woelk T, Puri C, Maspero E, Tacchetti C, Transidico P, Di Fiore PP, Polo S. Sigismund S, et al. Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):2760-5. doi: 10.1073/pnas.0409817102. Epub 2005 Feb 8. Proc Natl Acad Sci U S A. 2005. PMID: 15701692 Free PMC article.

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