Erratum: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Abstract

An erratum was issued for: Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. The Protocol was updated. Step 6.1.4.2 of the Protocol was updated from: If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously²⁰) using this equation: E = ((O-B) x V x D)/C where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B". Note: The predetermined constants (units OD600, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.052), A. pomorum (0.038), L. brevis (0.056), L. plantarum (0.077). to: If using the 4 species described here, normalize cells to equivalent colony forming unit (CFU)/ml densities (OD600 to CFU conversion determined previously²⁰) using this equation: E = ((O-B) x V x D)/C where E = volume to resuspend pellet in (μl), O = OD600 bacteria, B = OD600 blank media, D = fold-dilution, V = µl bacterial culture prior to centrifugation, C = OD600 of predetermined constant. See Supplemental Code File for examples of calculations using these equations. For spectrophotometers that automatically blank, use "O" in place of "O-B". Note: The predetermined constants (units OD600, normalized to 10⁷ CFU ml^-1, constants derived in²⁰) are as follows: A. tropicalis (0.053), A. pomorum (0.038), L. brevis (0.077), L. plantarum (0.056).