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. 1979 May 1;179(2):299-303.
doi: 10.1042/bj1790299.

Solubilization of human erythrocyte membrane glycoproteins by triton X-100

R S Pratt, G M Cook

Solubilization of human erythrocyte membrane glycoproteins by triton X-100

R S Pratt et al. Biochem J. .

Abstract

1. The enzymic removal of sialic acid residues from the glycoproteins of the human erythrocyte decreases the solubilization of membrane glycoprotein by Triton X-100. 2. The solubilization of asialoglycoprotein by Triton X-100 may be restored by the addition of borate. 3. Use of this non-ionic detergent in the presence of borate, as a general procedure for the mild solubilization of membrane glycoproteins deficient in sialic acid residues, is discussed.

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