doi: 10.1007/BF00267256.
Integration of IS3 into IS2 generates a short sequence duplication
- PMID: 395417
- DOI: 10.1007/BF00267256
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Integration of IS3 into IS2 generates a short sequence duplication
Mol Gen Genet.
1979.
Abstract
The Gal+ allele IS2-43 is known to segregate Gal- clones. Among 11 Gal- segregants, one was shown to be due to the integration of IS3 into IS2-43. Precise excision of the integrated IS3 element occurred at a rate of 5 x 10(-9)/cell/generation. DNA sequence analysis revealed that the termini of the IS3 element have the relation of imperfect inverted repeats and it is now flanked by a 3bp or 4bp duplication, a size which has not been seen before with other elements.
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