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. 1986 Jan;51(1):201-2.
doi: 10.1128/aem.51.1.201-202.1986.

Characteristics of methanogens isolated from bovine rumen

T L Miller, M J Wolin, H X Zhao, M P Bryant

Characteristics of methanogens isolated from bovine rumen

T L Miller et al. Appl Environ Microbiol. 1986 Jan.

Abstract

Six strains of methanogens were isolated from 10(-8) and 10(-9) ml of bovine rumen contents. All strains had the morphologic and physiologic characteristics of Methanobrevibacter spp. Four strains required coenzyme M; two did not. Growth of all strains either depended on or was stimulated by a mixture of isobutyric, isovaleric, 2-methylbutyric, and valeric acids. None of the strains reacted with antiserum against the type strain of Methanobrevibacter ruminantium.

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