Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Nov 16;50(1):11.
doi: 10.1007/s11064-024-04268-9.

Acute Behavioral and Neurochemical Effects of Sulpiride in Adult Zebrafish

David S Galstyan  1   2 , Andrey S Lebedev  1   2 , Nikita P Ilyin  1   2 , Maria S Papulova  1 , Nikita I Golushko  1 , Valeria V Tishkina  1 , Daryna K Saklakova  1 , Daniil Martynov  1   2 , Tatiana O Kolesnikova  3 , Dennis B Rosemberg  4 , Murilo S De Abreu  5   6 , Konstantin A Demin  7   8 , Allan V Kalueff  9   10   11
Affiliations

Acute Behavioral and Neurochemical Effects of Sulpiride in Adult Zebrafish

David S Galstyan et al. Neurochem Res. .

Abstract

Affective and psychotic disorders are highly prevalent and severely debilitating mental illnesses that often remain untreated or treatment-resistant. Sulpiride is a common antipsychotic (neuroleptic) drug whose well-established additional (e.g., antidepressant) therapeutic effects call for further studies of a wider spectrum of its CNS effects. Here, we examined effects of acute 20-min exposure to sulpiride (50-200 mg/L) on anxiety- and depression-like behaviors, as well as on brain monoamines, in adult zebrafish (Danio rerio). Overall, sulpiride exerted overt anxiolytic-like effects in the novel tank test and showed tranquilizing-like effects in the zebrafish tail immobilization test, accompanied by lowered whole-brain dopamine and its elevated turnover, without affecting serotonin or norepinephrine levels and their turnover. Taken together, these findings support complex behavioral pharmacology of sulpiride in vivo and reconfirm high sensitivity of zebrafish-based screens to this and, likely, other related clinically active neuroleptics.

Keywords: Anxiety; Behavioral despair; Depression; Dopamine; Monoamines; Sulpiride; Zebrafish.

PubMed Disclaimer

Conflict of interest statement

Declarations Ethical Approval Animal experiments were approved by the Institutional IACUC and fully adhered to the National and Institutional guidelines and regulations on animal experimentation, as well as the 3Rs principles of humane animal experimentation. Competing Interests The authors declare no competing interests.

References

    1. Nestler EJ, Hyman SE (2010) Animal models of neuropsychiatric disorders. Nat Neurosci 13(10):1161–1169 - PubMed - PMC
    1. Fried EI et al (2016) What are’good’depression symptoms? Comparing the centrality of DSM and non-DSM symptoms of depression in a network analysis. J Affect Disord 189:314–320 - PubMed
    1. Kanter JW et al (2008) The nature of clinical depression: symptoms, syndromes, and behavior analysis. Behav Analyst 31:1–21
    1. American Psychiatric Association, D. and, American Psychiatric D, Association (2013) Diagnostic and statistical manual of mental disorders: DSM-5, vol 5. American psychiatric association Washington, DC
    1. Ménard C, Hodes GE, Russo SJ (2016) Pathogenesis of depression: insights from human and rodent studies. Neuroscience 321:138–162 - PubMed

LinkOut - more resources