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. 2025 Jan 6;53(D1):D609-D617.
doi: 10.1093/nar/gkae1010.

UniProt: the Universal Protein Knowledgebase in 2025

UniProt Consortium
Collaborators

UniProt: the Universal Protein Knowledgebase in 2025

UniProt Consortium. Nucleic Acids Res. .

Abstract

The aim of the UniProt Knowledgebase (UniProtKB; https://www.uniprot.org/) is to provide users with a comprehensive, high-quality and freely accessible set of protein sequences annotated with functional information. In this publication, we describe ongoing changes to our production pipeline to limit the sequences available in UniProtKB to high-quality, non-redundant reference proteomes. We continue to manually curate the scientific literature to add the latest functional data and use machine learning techniques. We also encourage community curation to ensure key publications are not missed. We provide an update on the automatic annotation methods used by UniProtKB to predict information for unreviewed entries describing unstudied proteins. Finally, updates to the UniProt website are described, including a new tab linking protein to genomic information. In recognition of its value to the scientific community, the UniProt database has been awarded Global Core Biodata Resource status.

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Figures

Graphical Abstract
Graphical Abstract
Figure 1.
Figure 1.
The annotation of an extended-spectrum beta-lactamase (P28585) which confers resistance to penicillins.
Figure 2.
Figure 2.
Community submitted information is now added to relevant UniProtKB entries.
Figure 3.
Figure 3.
The Genomics Tab view for the human Tyrosine-protein kinase Lck protein (P06239).
Figure 4.
Figure 4.
Visualization of the RNase H2 complex as seen in the UniProt entry for the Ribonuclease H2 subunit A protein (O75792).
See this image and copyright information in PMC

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