Effect of cytosine arabinoside on the composition of the nonneuronal cell population in cerebral explants
- PMID: 3956670
- DOI: 10.1016/0014-4886(86)90092-0
Effect of cytosine arabinoside on the composition of the nonneuronal cell population in cerebral explants
Abstract
Rat cortical explants were cultured in the presence or absence of the mitotic inhibitor cytosine arabinoside to determine whether or not it affects the composition of the nonneuronal cell population within the outgrowth zone. Ultrastructural morphometric analysis of the incidence of fibroblasts, fibrous astrocytes, and protoplasmic astrocytes-epithelial cells at 18 days in vitro, revealed statistically significant decreases in the incidence of fibroblasts and fibrous astrocytes in the explants treated with the inhibitor compared with control explants. Coupled with earlier findings of enhanced neurite outgrowth and decreased nonneuronal cell proliferation that follows such treatment, it appears that cytosine arabinoside may potentiate neurite outgrowth by altering the composition, as well as the number, of nonneuronal cells in the outgrowth zone. These data indicate that fibroblasts and fibrous astrocytes may limit the regenerative response of severed axons in the mammalian central nervous system.
Similar articles
-
Effect of fluorodeoxyuridine on neurons and non-neuronal cells in cerebral explants.Exp Brain Res. 1989;78(1):132-8. doi: 10.1007/BF00230692. Exp Brain Res. 1989. PMID: 2531679
-
Effect of cytosine arabinoside on rat cerebral explants: fibroblasts and reactive microglial cells as the primary cellular barrier to neurite growth.Neurosci Lett. 1989 Jul 31;102(2-3):332-6. doi: 10.1016/0304-3940(89)90101-8. Neurosci Lett. 1989. PMID: 2812510
-
Neuronal survival and neurite growth in cultured cerebral explants: assessment of the effect of cytosine arabinoside using improved stereology.Brain Res. 1991 Apr 12;546(1):146-50. doi: 10.1016/0006-8993(91)91170-6. Brain Res. 1991. PMID: 1855145
-
Non-neuronal cell proliferation in tissue culture: implications for axonal regeneration in the central nervous system.Brain Res. 1986 Mar 12;368(1):49-61. doi: 10.1016/0006-8993(86)91041-3. Brain Res. 1986. PMID: 3513901
-
Neurite growth from, and neuronal survival within, cultured explants of the nervous system: a critical review of morphometric and stereological methods, and suggestions for the future.Prog Neurobiol. 1991;37(6):525-46. doi: 10.1016/0301-0082(91)90007-n. Prog Neurobiol. 1991. PMID: 1763189 Review. No abstract available.
Cited by
-
Adjusting the neuron to astrocyte ratio with cytostatics in hippocampal cell cultures from postnatal rats: A comparison of cytarabino furanoside (AraC) and 5-fluoro-2'-deoxyuridine (FUdR).PLoS One. 2022 Mar 9;17(3):e0265084. doi: 10.1371/journal.pone.0265084. eCollection 2022. PLoS One. 2022. PMID: 35263366 Free PMC article.
-
Effect of fluorodeoxyuridine on neurons and non-neuronal cells in cerebral explants.Exp Brain Res. 1989;78(1):132-8. doi: 10.1007/BF00230692. Exp Brain Res. 1989. PMID: 2531679
-
Cytosolic Escape of Mitochondrial DNA Triggers cGAS-STING Pathway-Dependent Neuronal PANoptosis in Response to Intermittent Hypoxia.Neurochem Res. 2024 Aug;49(8):2228-2248. doi: 10.1007/s11064-024-04151-7. Epub 2024 Jun 4. Neurochem Res. 2024. PMID: 38833090
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources