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. 2024 Nov 21;11(1):1267.
doi: 10.1038/s41597-024-04118-x.

Expanding the genome information on Bacillales for biosynthetic gene cluster discovery

Lijie Song #  1 , Lasse Johan Dyrbye Nielsen #  1 , Xinming Xu  1   2 , Omkar Satyavan Mohite  3 , Matin Nuhamunada  3 , Zhihui Xu  4 , Rob Murphy  5 , Kasun Bodawatta  6   7 , Michael Poulsen  5 , Mohamed Hatha Abdulla  8 , Eva C Sonnenschein  1   9 , Tilmann Weber  10 , Ákos T Kovács  11   12
Affiliations

Expanding the genome information on Bacillales for biosynthetic gene cluster discovery

Lijie Song et al. Sci Data. .

Abstract

This study showcases 121 new genomes of spore-forming Bacillales from strains collected globally from a variety of habitats, assembled using Oxford Nanopore long-read and MGI short-read sequences. Bacilli are renowned for their capacity to produce diverse secondary metabolites with use in agriculture, biotechnology, and medicine. These secondary metabolites are encoded within biosynthetic gene clusters (smBGCs). smBGCs have significant research interest due to their potential as sources of new bioactivate compounds. Our dataset includes 62 complete genomes, 2 at chromosome level, and 57 at contig level, covering a genomic size range from 3.50 Mb to 7.15 Mb. Phylotaxonomic analysis revealed that these genomes span 16 genera, with 69 of them belonging to Bacillus. A total of 1,176 predicted BGCs were identified by in silico genome mining. We anticipate that the open-access data presented here will expand the reported genomic information of spore-forming Bacillales and facilitate a deeper understanding of the genetic basis of Bacillales' potential for secondary metabolite production.

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Conflict of interest statement

Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Distribution of sample collection site coordinates depicted using OpenStreetMap.
Fig. 2
Fig. 2
Illustration of Genome assembly and BGC analysis. (a) Strategy for sequencing and genome assembly, (b) the BGC analysis pipeline.
Fig. 3
Fig. 3
A phylogenetic tree of the 121 genomes with plasmid content and BGC class and count indicated.
Fig. 4
Fig. 4
The number of BGCs in each genus of the 121 genomes.
See this image and copyright information in PMC

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