doi: 10.1038/s41375-024-02478-2.
Epub 2024 Nov 22.
A DNA methylation database of human and mouse hematological malignancy cell lines
Affiliations
- PMID: 39578534
- PMCID: PMC11794145
- DOI: 10.1038/s41375-024-02478-2
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A DNA methylation database of human and mouse hematological malignancy cell lines
Leukemia.
2025 Feb.
No abstract available
Conflict of interest statement
Competing interests: ME declares past grants from Ferrer International and Incyte and personal fees from Quimatryx, outside the submitted work.
Figures
A Schematic representation of hierarchical subdivision of human (top) and mouse (bottom) cell line cohorts based on hematological malignancies. B Heatmap resulting from the unsupervised analysis of the human (left) and mouse (right) hematological cell lines. Dendogram clustering was performed using all methylation values, an 1% of randomly selected CpG probes were used for heatmap visualization. C Phylogenetic analysis of the different hematological subtypes and species. Each leaf represents the 1% most variable CpG sites within each hematologic subtype and specie. The inner circle colors represent if the leaf corresponds to leukemia, lymphoma, multiple myeloma or transformed cell lines, while the outer circle corresponds to the refined classification for human hematological cell lines. D Heatmap resulting from the differential methylation analysis within leukemia, lymphoma and multiple myeloma in human (left) and mouse (right) hematological cell lines. The bottom annotation indicates hematological disease, as described in the heatmap legend. Methylation β-values range from 0 (green) to 1 (red).
A Heatmap resulting from the CpG sites differentially methylated within hematological diseases in human hematological cell lines. The bottom annotation indicates hematological disease, as described in the heatmap legend. Methylation β-values range from 0 (green) to 1 (red). B Heatmap resulting from the CpG sites differentially methylated within hematological diseases in human primary samples. The bottom annotation indicates hematological disease, as described in the heatmap legend. Methylation β-values range from 0 (green) to 1 (red). C Heatmap resulting from the common CpG sites associated with cytarabine, fludarabine and nelarabine drug sensitivity. Top annotation indicates whether the cells are in the sensitive cluster or resistant cluster. The bottom annotation indicates hematological disease and cytarabine, fludarabine and nelarabine sensitivity as described in the heatmap legend. Methylation β-values range from 0 (green) to 1 (red). D Boxplot representing cytarabine (top), fludarabine (middle) and nelarabine (bottom) IC50s of human hematological cell lines grouped according to whether they clustered in sensitive or resistant cluster. Two-sided Mann–Whitney–Wilcoxon test was performed.
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