Effect of salinity on nitrogen removal performance, enzymatic activity and metabolic pathway of Chlorella pyrenoidosa treating aquaculture wastewater

Abstract

The nitrogen removal performance, enzymatic activity, antioxidant response and metabolic pathway of Chlorella pyrenoidosa (C. pyrenoidosa) under different salinities have been investigated during the treatment of aquaculture wastewater. The growth, chlorophyll content and photosynthetic activity of C. pyrenoidosa were negatively correlated with the salinity from 1% to 3%. The removal performance of chemical oxygen demand (COD) and nitrogen compounds for C. pyrenoidosa decreased with the increase of salinity from 1% to 3%, which was due to the decrease of their corresponding metabolism enzymatic activities. The equilibrium between the reactive oxygen species production and antioxidant defensive system in C. pyrenoidosa was destroyed under high salinity stress and then caused an irreversible damage, which decreased the nitrogen assimilation of C. pyrenoidosa. The metabolic pathway of C. pyrenoidosa under 3% salinity had some obvious variation by comparison with 1% salinity, which led to the discrepancy in the microalgae activity and nitrogen transformation performance. Additionally, high salinity could inhibit the expression of gene associated with the chlorophyll synthesis and damaged the photosystem II reaction center. This study can provide an insight into the effect of salinity on the nitrogen removal from aquaculture wastewater by microalgae.

Keywords: Aquaculture wastewater; Metabolic pathway; Nitrogen metabolism; Reactive oxygen species; Salinity.