Exploring perfluoroalkyl substances contamination in human breast milk: First ghanaian study

Abstract

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) represent a category of synthetic organic chemical contaminants that have garnered increasing attention due to their potential adverse impacts. Existing research underscores the contamination of human breast milk by PFAS, raising concerns regarding potential deleterious health effects in children. The study aimed to explore the levels of some PFAS in human breast milk in a previously unstudied population to determine the extent of infants' exposure. The research was conducted at Ho Teaching Hospital in Ghana. The study protocol was reviewed and approval by University of Health and Allied Sciences Ethics Research Committee (UHAS-REC). Twenty-nine (29) mothers, aged 18-44 years, were enrolled in the research. Sample collection spanned from December 28, 2020 to June 30, 2021. Ten millilitres (10 mL) of breast milk were collected from each participant into cleaned bottles from day of birth to after two weeks postpartum, following a standardized protocol and stored at -20 °C. Sample preparation and analysis employed solid phase extraction methodology. Subsequently, the processed extracts were subjected to analysis using ultra-high-performance liquid chromatography (UPLC-MS/MS). The data obtained were analysed using IBM SPSS Statistics version 26, Excel 2016, and Xlstat 2022. Descriptive statistics were employed to summarize the study variables. The mean/mode input method was used to treat missing data. The median and interquartile range (IQR) of PFAS concentrations in the breast milk were: PFHxA, 6.0 ng/L(IQR, 2.2 ng/L), PFHpA, 5.6 ng/L(IQR, 2.1 ng/L), PFOA, 72.0 ng/L(IQR, 16.0 ng/L), and PFOS, 93.0 ng/L(IQR, 8.0 ng/L) ng/L) respectively. PFOS and PFOA were the most dominant PFAS in the breast milk which is consistent with worldwide reports. The levels of PFAS, particularly PFOS and PFOA, in breast milk points to seemingly high levels of PFAS exposure and contamination of mothers and neonates in the region.

Keywords: Human breast milk; PFAS; PFHpA; PFHxA; PFOA; PFOS.