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. 2024 Nov 23;82(1):6.
doi: 10.1007/s00284-024-03952-x.

Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater

Shijie Ou #  1   2 , Xinyi Tian #  2 , Zhen Zhang  2 , Lingjia Zhu  3 , Rong Wang  2 , Guangxiang Cao  1   2 , Jiafang Fu  4   5 , Peipei Zhang  6   7
Affiliations

Characterization of a Novel Tn7-like Transposon Carrying blaDHA-1 in Providencia stuartii MF1 Isolated from Swine Wastewater

Shijie Ou et al. Curr Microbiol. .

Abstract

Providencia stuartii is an emerging pathogen that causes nosocomial infections. In this study, a multidrug-resistant strain P. stuartii MF1 was isolated from swine wastewater. Comprehensive analysis of whole genome sequencing revealed that dozens of antibiotic resistance genes were found in MF1. A novel transposon Tn6450M which has high sequence identity to Tn6450 and the plasmid-borne Tn6765 from Proteus mirabilis was identified in the genome of MF1. Tn6450M was determined to be stably inserted into a new attTn7 site in the P. stuartii MF1 genome and contains the third-generation cephalosporins resistance-associated genes blaDHA-1. Intergeneric transmission of Tn6450 variants poses risks for the spread of antibiotic resistance genes.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that there is no conflict of interest regarding the publication of this article.

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