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. 2024 Nov 11:37:12979.
doi: 10.3389/ti.2024.12979. eCollection 2024.

The impact of the EVLP on the lung microbiome and its inflammatory reaction

Leandro Grando  1 , Marc Boada  1 , Rosa Faner  2   3   4   5 , Susana Gómez-Ollés  3   6 , Victoria Ruiz  6 , Marc Bohils  7 , Joaquim Albiol  7 , Ramses Marrero  8 , Laia Rosell  9 , Ivan Salinas  9 , Daniel Ruiz  9 , Ángel Ruiz  7 , Camino Rodríguez-Villar  7 , Anna Ureña  1 , David Paredes-Zapata  7 , Ángela Guirao  1 , Gerard Sánchez-Etayo  7 , Laureano Molins  1 , Néstor Quiroga  1 , Aroa Gómez-Brey  10 , Xavier Michavila  1 , Alberto Sandiumenge  10 , Àlvar Agustí  2   3   11 , Ricard Ramos  1   4 , Irene Bello  1   4
Affiliations

The impact of the EVLP on the lung microbiome and its inflammatory reaction

Leandro Grando et al. Transpl Int. .

Abstract

The pulmonary microbiome has emerged as a significant factor in respiratory health and diseases. Despite the sterile conditions maintained during ex vivo lung perfusion (EVLP), the use of antibiotics in the perfuse liquid can lead to dynamic changes in the lung microbiome. Here, we present the design of a study that aims to investigate the hypothesis that EVLP alters the lung microbiome and induces tissue inflammation. This pilot, prospective, controlled study will be conducted in two Spanish donor centers and will include seven organ donors after brain death or after controlled cardiac death. After standardized retrieval, the left lung will be preserved in cold storage and the right lung will be perfused with EVLP. Samples from bronchoalveolar lavage, perfusion and preservation solutions, and lung biopsies will be collected from both lungs and changes in lung microbiome and inflammatory response will be compared.

Keywords: EVLP; ex vivo lung perfusion; inflammatory response; lung donor; lung microbiome; primary graft dysfunction; protocol study.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Protocol scheme.
See this image and copyright information in PMC

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